OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 1 — Jan. 4, 2010

Intravital imaging of amyloid plaques in a transgenic mouse model using optical-resolution photoacoustic microscopy

Song Hu, Ping Yan, Konstantin Maslov, Jin-Moo Lee, and Lihong V. Wang  »View Author Affiliations


Optics Letters, Vol. 34, Issue 24, pp. 3899-3901 (2009)
http://dx.doi.org/10.1364/OL.34.003899


View Full Text Article

Enhanced HTML    Acrobat PDF (304 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We report optical-resolution photoacoustic microscopy (OR-PAM) for in vivo imaging of amyloid plaques in an Alzheimer’s disease mouse model. Validation using conventional fluorescence microscopy and multiphoton microscopy shows that OR-PAM has sufficient sensitivity and spatial resolution to identify amyloid plaques in living brains. In addition, with dual-wavelength OR-PAM, the three-dimensional morphology of amyloid plaques and the surrounding microvasculature are imaged simultaneously through a cranial window without angiographic contrast agents. OR-PAM, capable of providing both exogenous molecular contrast and endogenous hemoglobin contrast, has the potential to serve as a new technology for in vivo microscopic observations of cerebral plaque deposits.

© 2009 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: July 8, 2009
Revised Manuscript: November 10, 2009
Manuscript Accepted: November 10, 2009
Published: December 15, 2009

Virtual Issues
Vol. 5, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Song Hu, Ping Yan, Konstantin Maslov, Jin-Moo Lee, and Lihong V. Wang, "Intravital imaging of amyloid plaques in a transgenic mouse model using optical-resolution photoacoustic microscopy," Opt. Lett. 34, 3899-3901 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-34-24-3899


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. C. Wu, V. W. Pike, and Y. Wang, Curr. Top Dev. Biol. 70, 171 (2005). [CrossRef] [PubMed]
  2. W. E. Klunk, B. J. Bacskai, C. A. Mathis, S. T. Kajdasz, M. E. McLellan, M. P. Frosch, M. L. Debnath, D. P. Holt, Y. Wang, and B. T. Hyman, J. Neuropathol. Exp. Neurol. 61, 797 (2002). [PubMed]
  3. S. Hu, K. Maslov, and L. V. Wang, Med. Phys. 36, 2320 (2009). [CrossRef] [PubMed]
  4. C. L. Nie, X. S. Wang, Y. Liu, S. Perrett, and R. Q. He, BMC Neurosci. 8, 9 (2007). [CrossRef] [PubMed]
  5. S. L. Jacques and S. A. Prahl, http://omlc.ogi.edu/spectra/hemoglobin/index. html, retrieved November 16, 2009.
  6. S. Hu, K. Maslov, V. Tsytsarev, and L. V. Wang, J. Biomed. Opt. 14, 040503 (2009). [CrossRef] [PubMed]
  7. M. Hintersteiner, A. Enz, P. Frey, A. L. Jaton, W. Kinzy, R. Kneuer, U. Neumann, M. Rudin, M. Staufenbiel, M. Stoeckli, K. H. Wiederhold, and H. U. Gremlich, Nat. Biotechnol. 23, 577 (2005). [CrossRef] [PubMed]
  8. H. T. Xu, F. Pan, G. Yang, and W. B. Gan, Nat. Neurosci. 10, 549 (2007). [CrossRef] [PubMed]
  9. P. Yan, A. W. Bero, J. R. Cirrito, Q. Xiao, X. Hu, Y. Wang, E. Gonzales, D. M. Holtzman, and J. M. Lee, J. Neurosci. 29, 10706 (2009). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 

Supplementary Material


» Media 1: AVI (4634 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited