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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 5 — May. 5, 2009

Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection

Jonas Kühn, Frédéric Montfort, Tristan Colomb, Benjamin Rappaz, Corinne Moratal, Nicolas Pavillon, Pierre Marquet, and Christian Depeursinge  »View Author Affiliations


Optics Letters, Vol. 34, Issue 5, pp. 653-655 (2009)
http://dx.doi.org/10.1364/OL.34.000653


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Abstract

We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally spaced in spatial frequency in the 485 670 nm range, we are able to achieve a slice-by-slice tomographic reconstruction with a 0.6 1 μ m axial resolution in a biological medium. The method is applied to erythrocytes investigation to retrieve the cellular membrane profile in three dimensions.

© 2009 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.6960) Medical optics and biotechnology : Tomography
(090.1995) Holography : Digital holography
(110.6955) Imaging systems : Tomographic imaging

ToC Category:
Imaging Systems

History
Original Manuscript: November 10, 2008
Revised Manuscript: January 15, 2009
Manuscript Accepted: January 16, 2009
Published: February 25, 2009

Virtual Issues
Vol. 4, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Jonas Kühn, Frédéric Montfort, Tristan Colomb, Benjamin Rappaz, Corinne Moratal, Nicolas Pavillon, Pierre Marquet, and Christian Depeursinge, "Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection," Opt. Lett. 34, 653-655 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-34-5-653

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