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Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 5 — May. 5, 2009

Fast two-dimensional standing-wave total-internal-reflection fluorescence microscopy using acousto-optic deflectors

Olga Gliko, William E. Brownell, and Peter Saggau  »View Author Affiliations

Optics Letters, Vol. 34, Issue 6, pp. 836-838 (2009)

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We report a scheme for 2D standing-wave total-internal-reflection fluorescence microscopy. Standing-wave patterns are generated by two interfering beams coupled through the objective lens. Period, angular orientation, and phase of standing waves are controlled entirely by acousto-optic deflectors. The lateral resolution improvement of 100 nm is combined with an axial selectivity of < 100 nm by utilizing an evanescent standing-wave pattern. This technique can provide real-time imaging of subresolution structures in live biological specimens near a glass–water interface.

© 2009 Optical Society of America

OCIS Codes
(050.5080) Diffraction and gratings : Phase shift
(110.4850) Imaging systems : Optical transfer functions
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(180.2520) Microscopy : Fluorescence microscopy
(230.1040) Optical devices : Acousto-optical devices
(110.3010) Imaging systems : Image reconstruction techniques

ToC Category:

Original Manuscript: October 23, 2008
Revised Manuscript: January 15, 2009
Manuscript Accepted: January 29, 2009
Published: March 12, 2009

Virtual Issues
Vol. 4, Iss. 5 Virtual Journal for Biomedical Optics

Olga Gliko, William E. Brownell, and Peter Saggau, "Fast two-dimensional standing-wave total-internal-reflection fluorescence microscopy using acousto-optic deflectors," Opt. Lett. 34, 836-838 (2009)

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