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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 5 — May. 5, 2009

Sizing of particles smaller than 5 μ m in digital holographic microscopy

Xuecheng Wu, Gérard Gréhan, Siegfried Meunier-Guttin-Cluzel, Linghong Chen, and Kefa Cen  »View Author Affiliations

Optics Letters, Vol. 34, Issue 6, pp. 857-859 (2009)

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The holographic microscopy technique is a strong contender for dynamic three-dimensional (3D) measurement of small particles (typically smaller than 5 μ m ) in microchannels. However, there is a big challenge to accurately measure the size of such small particles. The traditional hologram reconstruction method was numerically investigated. It is found that the error level, especially for the size measurement, is higher than expected, even in an ideal situation without consideration of noise. An alternative way based on Lorenz–Mie (LM) calculations was then presented. The intensity distribution of the fringe pattern on the particle hologram is directly used and compared with the ones calculated using an LM-based program. Various cases for particle sizes from 0.5 to 5 μ m and recording distances from 5 to 500 μ m are tested. The results show that the accuracy in particle sizing can be significantly improved.

© 2009 Optical Society of America

OCIS Codes
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(290.4020) Scattering : Mie theory
(350.4990) Other areas of optics : Particles
(090.1995) Holography : Digital holography

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Original Manuscript: December 12, 2008
Revised Manuscript: February 3, 2009
Manuscript Accepted: February 8, 2009
Published: March 13, 2009

Virtual Issues
Vol. 4, Iss. 5 Virtual Journal for Biomedical Optics

Xuecheng Wu, Gérard Gréhan, Siegfried Meunier-Guttin-Cluzel, Linghong Chen, and Kefa Cen, "Sizing of particles smaller than 5 μm in digital holographic microscopy," Opt. Lett. 34, 857-859 (2009)

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