Subwavelength-resolution label-free photoacoustic microscopy of optical absorption in vivo
Optics Letters, Vol. 35, Issue 19, pp. 3195-3197 (2010)
http://dx.doi.org/10.1364/OL.35.003195
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Abstract
Optical absorption provides essential biological functional information but cannot be sensed by mainstream optical microscopy technologies directly, which detect fluorescence or scattering and may require undesirable labeling. Here we developed in vivo subwavelength-resolution photoacoustic microscopy (SW-PAM) that provides exquisitely high optical-absorption contrast due to nonfluorescent, or fluorescent, endogenous pigments. Having approached the ultimate diffraction-limited optical resolution, SW-PAM can resolve subcellular organelles. Vasculature and early-stage melanoma were imaged with
© 2010 Optical Society of America
OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(180.5810) Microscopy : Scanning microscopy
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: July 26, 2010
Revised Manuscript: August 27, 2010
Manuscript Accepted: August 31, 2010
Published: September 21, 2010
Virtual Issues
Vol. 5, Iss. 14 Virtual Journal for Biomedical Optics
Citation
Chi Zhang, Konstantin Maslov, and Lihong V. Wang, "Subwavelength-resolution label-free photoacoustic microscopy of optical absorption in vivo," Opt. Lett. 35, 3195-3197 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-35-19-3195
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