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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 5, Iss. 14 — Nov. 16, 2010

Subwavelength-resolution label-free photoacoustic microscopy of optical absorption in vivo

Chi Zhang, Konstantin Maslov, and Lihong V. Wang  »View Author Affiliations

Optics Letters, Vol. 35, Issue 19, pp. 3195-3197 (2010)

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Optical absorption provides essential biological functional information but cannot be sensed by mainstream optical microscopy technologies directly, which detect fluorescence or scattering and may require undesirable labeling. Here we developed in vivo subwavelength-resolution photoacoustic microscopy (SW-PAM) that provides exquisitely high optical-absorption contrast due to nonfluorescent, or fluorescent, endogenous pigments. Having approached the ultimate diffraction-limited optical resolution, SW-PAM can resolve subcellular organelles. Vasculature and early-stage melanoma were imaged with 12 : 1 and 17 : 1 contrasts, respectively, without labeling. SW-PAM along with the scaled-up macroscopy, as the only technology that measures the same contrast origin over such a wide length scale, can potentially accelerate translation from microscopic research to clinical practice.

© 2010 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(180.5810) Microscopy : Scanning microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: July 26, 2010
Revised Manuscript: August 27, 2010
Manuscript Accepted: August 31, 2010
Published: September 21, 2010

Virtual Issues
Vol. 5, Iss. 14 Virtual Journal for Biomedical Optics

Chi Zhang, Konstantin Maslov, and Lihong V. Wang, "Subwavelength-resolution label-free photoacoustic microscopy of optical absorption in vivo," Opt. Lett. 35, 3195-3197 (2010)

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