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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 7 — Jul. 27, 2011

Label-free flow cytometry using multiplex coherent anti-Stokes Raman scattering (MCARS) for the analysis of biological specimens

Charles H. Camp, Jr., Siva Yegnanarayanan, Ali A. Eftekhar, and Ali Adibi  »View Author Affiliations


Optics Letters, Vol. 36, Issue 12, pp. 2309-2311 (2011)
http://dx.doi.org/10.1364/OL.36.002309


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Abstract

We present the first demonstration, to our knowledge, of a label-free flow cytometer for the analysis of biological specimens using multiplex coherent anti-Stokes Raman scattering (MCARS) and elastic scatter measurements. The MCARS system probes the Raman vibrational energy levels and the elastic scatter provides morphological information. We demonstrate these capabilities by probing a culture of Saccharomyces cerevisiae at 100 spectra / s and constructing a background-free Raman reconstruction using a Kramers–Kronig relation. A theoretical analysis shows that this system could operate at speeds of 10 kHz with appropriate hardware; thus facilitating integration into commercial flow cytometers or use as a high-speed, stand-alone device.

© 2011 Optical Society of America

OCIS Codes
(190.4380) Nonlinear optics : Nonlinear optics, four-wave mixing
(190.7110) Nonlinear optics : Ultrafast nonlinear optics
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering

ToC Category:
Nonlinear Optics

History
Original Manuscript: April 5, 2011
Revised Manuscript: May 11, 2011
Manuscript Accepted: May 18, 2011
Published: June 14, 2011

Virtual Issues
Vol. 6, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Charles H. Camp, Jr., Siva Yegnanarayanan, Ali A. Eftekhar, and Ali Adibi, "Label-free flow cytometry using multiplex coherent anti-Stokes Raman scattering (MCARS) for the analysis of biological specimens," Opt. Lett. 36, 2309-2311 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-36-12-2309

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