Quantitative phase recovery of phase objects is achieved by a direct inversion using the defocused weak object transfer function. The presented method is noniterative and is based on partially coherent principles. It also takes into account the optical properties of the system and gives the phase of the object directly. The proposed method is especially suitable for application to weak phase objects, such as live and unstained biological samples but, surprisingly, has also been shown to work with comparatively strong phase objects.
© 2011 Optical Society of America
Original Manuscript: February 14, 2011
Revised Manuscript: June 9, 2011
Manuscript Accepted: June 14, 2011
Published: July 13, 2011
Vol. 6, Iss. 8 Virtual Journal for Biomedical Optics
Shan Shan Kou, Laura Waller, George Barbastathis, Pierre Marquet, Christian Depeursinge, and Colin J. R. Sheppard, "Quantitative phase restoration by direct inversion using the optical transfer function," Opt. Lett. 36, 2671-2673 (2011)