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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 9 — Oct. 3, 2011

Noninvasive spectral imaging of skin chromophores based on multiple regression analysis aided by Monte Carlo simulation

Izumi Nishidate, Aditya Wiswadarma, Yota Hase, Noriyuki Tanaka, Takaaki Maeda, Kyuichi Niizeki, and Yoshihisa Aizu  »View Author Affiliations

Optics Letters, Vol. 36, Issue 16, pp. 3239-3241 (2011)

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In order to visualize melanin and blood concentrations and oxygen saturation in human skin tissue, a simple imaging technique based on multispectral diffuse reflectance images acquired at six wavelengths (500, 520, 540, 560, 580 and 600 nm ) was developed. The technique utilizes multiple regression analysis aided by Monte Carlo simulation for diffuse reflectance spectra. Using the absorbance spectrum as a response variable and the extinction coefficients of melanin, oxygenated hemoglobin, and deoxygenated hemoglobin as predictor variables, multiple regression analysis provides regression coefficients. Concentrations of melanin and total blood are then determined from the regression coefficients using conversion vectors that are deduced numerically in advance, while oxygen saturation is obtained directly from the regression coefficients. Experiments with a tissue-like agar gel phantom validated the method. In vivo experiments with human skin of the human hand during upper limb occlusion and of the inner forearm exposed to UV irradiation demonstrated the ability of the method to evaluate physiological reactions of human skin tissue.

© 2011 Optical Society of America

OCIS Codes
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: April 26, 2011
Revised Manuscript: July 6, 2011
Manuscript Accepted: July 20, 2011
Published: August 15, 2011

Virtual Issues
Vol. 6, Iss. 9 Virtual Journal for Biomedical Optics

Izumi Nishidate, Aditya Wiswadarma, Yota Hase, Noriyuki Tanaka, Takaaki Maeda, Kyuichi Niizeki, and Yoshihisa Aizu, "Noninvasive spectral imaging of skin chromophores based on multiple regression analysis aided by Monte Carlo simulation," Opt. Lett. 36, 3239-3241 (2011)

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