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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 7 — Jun. 25, 2012

Targeted alteration of real and imaginary refractive index of biological cells by histological staining

L. Cherkezyan, H. Subramanian, V. Stoyneva, J. D. Rogers, S. Yang, D. Damania, A. Taflove, and V. Backman  »View Author Affiliations

Optics Letters, Vol. 37, Issue 10, pp. 1601-1603 (2012)

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Various staining techniques are commonly used in biomedical research to investigate cellular morphology. By inducing absorption of light, staining dyes change the intracellular refractive index due to the Kramers–Kronig relationship. We present a method for creating 2D maps of real and imaginary refractive indices of stained biological cells using their thickness and absorptance. We validate our technique on dyed polystyrene microspheres and quantify the alteration in refractive index of stained biological cells. We reveal that specific staining of individual organelles can increase their scattering cross-section by orders of magnitudes, implying a major impact in the field of biophotonics.

© 2012 Optical Society of America

OCIS Codes
(160.4760) Materials : Optical properties
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(290.0290) Scattering : Scattering
(300.6550) Spectroscopy : Spectroscopy, visible

ToC Category:

Original Manuscript: December 2, 2011
Revised Manuscript: January 27, 2012
Manuscript Accepted: February 11, 2012
Published: May 9, 2012

Virtual Issues
Vol. 7, Iss. 7 Virtual Journal for Biomedical Optics

L. Cherkezyan, H. Subramanian, V. Stoyneva, J. D. Rogers, S. Yang, D. Damania, A. Taflove, and V. Backman, "Targeted alteration of real and imaginary refractive index of biological cells by histological staining," Opt. Lett. 37, 1601-1603 (2012)

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  1. E. D. P. De Robertis, F. A. Saez, and E. M. F. De Robertis, Cell Biology, 6th ed. (Saunders, 1975).
  2. E. S. Cibas and B. S. Ducatman, Cytology: Diagnostic Principles and Clinical Correlates (Saunders, 1996).
  3. J. D. Wilson, W. J. Cottrell, and T. H. Foster, J. Biomed. Opt. 12, 014010 (2007). [CrossRef]
  4. R. d. L. Kronig, J. Opt. Soc. Am. 12, 547 (1926). [CrossRef]
  5. H. A. Kramers, Atti del Congresso Internazionale dei Fisici 2, 545 (1927). [CrossRef]
  6. S. Uttam, R. K. Bista, D. J. Hartman, R. E. Brand, and Y. Liu, J. Biomed. Opt. 16, 116013 (2011). [CrossRef]
  7. V. Lucarini, Kramers-Kronig Relations in Optical Materials Research (Springer, 2005).
  8. H. C. v. d. Hulst, Light Scattering by Small Particles (Wiley, 1957).
  9. S. A. Prahl, “Inverse adding-doubling software,” http://omlc.ogi.edu/software/iad/index.html .
  10. J. W. Pickering, S. A. Prahl, N. Vanwieringen, J. F. Beek, H. J. C. M. Sterenborg, and M. J. C. Vangemert, Appl. Opt. 32, 399 (1993). [CrossRef]
  11. S. A. Prahl, M. J. C. Vangemert, and A. J. Welch, Appl. Opt. 32, 559 (1993). [CrossRef]
  12. C. Maetzler, “MATLAB functions for Mie scattering and absorption,” Research Report 2002-08 (Bern, 2002).
  13. D. J. Cook, in Cellular Pathology, 2nd ed. (Scion, 2006), pp. 67–104.
  14. G. C. Crossmon, Stain Technol. 24, 241 (1949). [CrossRef]
  15. H. G. Davies, M. H. F. Wilkins, J. Chayen, and L. F. Lacour, Q. J. Microsc. Sci. 95, 271 (1954).
  16. J. M. Schmitt and G. Kumar, Appl. Opt. 37, 2788 (1998). [CrossRef]
  17. G. W. Kattawar and G. N. Plass, Appl. Opt. 6, 1377(1967). [CrossRef]
  18. N. N. Boustany, S. A. Boppart, and V. Backman, Annu. Rev. Biomed. Eng. 12, 285 (2010). [CrossRef]
  19. H. Subramanian, P. Pradhan, Y. Liu, I. R. Capoglu, J. D. Rogers, H. K. Roy, R. E. Brand, and V. Backman, Opt. Lett. 34, 518 (2009). [CrossRef]

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