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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 7 — Jun. 25, 2012

Targeted alteration of real and imaginary refractive index of biological cells by histological staining

L. Cherkezyan, H. Subramanian, V. Stoyneva, J. D. Rogers, S. Yang, D. Damania, A. Taflove, and V. Backman  »View Author Affiliations


Optics Letters, Vol. 37, Issue 10, pp. 1601-1603 (2012)
http://dx.doi.org/10.1364/OL.37.001601


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Abstract

Various staining techniques are commonly used in biomedical research to investigate cellular morphology. By inducing absorption of light, staining dyes change the intracellular refractive index due to the Kramers–Kronig relationship. We present a method for creating 2D maps of real and imaginary refractive indices of stained biological cells using their thickness and absorptance. We validate our technique on dyed polystyrene microspheres and quantify the alteration in refractive index of stained biological cells. We reveal that specific staining of individual organelles can increase their scattering cross-section by orders of magnitudes, implying a major impact in the field of biophotonics.

© 2012 Optical Society of America

OCIS Codes
(160.4760) Materials : Optical properties
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(290.0290) Scattering : Scattering
(300.6550) Spectroscopy : Spectroscopy, visible

ToC Category:
Materials

History
Original Manuscript: December 2, 2011
Revised Manuscript: January 27, 2012
Manuscript Accepted: February 11, 2012
Published: May 9, 2012

Virtual Issues
Vol. 7, Iss. 7 Virtual Journal for Biomedical Optics

Citation
L. Cherkezyan, H. Subramanian, V. Stoyneva, J. D. Rogers, S. Yang, D. Damania, A. Taflove, and V. Backman, "Targeted alteration of real and imaginary refractive index of biological cells by histological staining," Opt. Lett. 37, 1601-1603 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-37-10-1601

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