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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 8 — Aug. 2, 2012

Quantitative phase microscopy of biological samples using a portable interferometer

Natan T. Shaked  »View Author Affiliations


Optics Letters, Vol. 37, Issue 11, pp. 2016-2018 (2012)
http://dx.doi.org/10.1364/OL.37.002016


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Abstract

This Letter presents the τ interferometer, a portable and inexpensive device for obtaining spatial interferograms of microscopic biological samples without the strict stability and the highly coherent illumination that are usually required for interferometric microscopy setups. The device is built using off-the-shelf optical elements and can easily operate with low-coherence illumination, while being positioned in the output of a conventional inverted microscope. The interferograms are processed into the quantitative amplitude and phase profiles of the sample. Based on the phase profile, the optical-path-delay profile is obtained with temporal stability of 0.18 nm and spatial stability of 0.42 nm. Further experimental demonstration of using the τ interferometer for imaging the quantitative thickness profile of a live red blood cell is provided.

© 2012 Optical Society of America

OCIS Codes
(090.2880) Holography : Holographic interferometry
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.3170) Microscopy : Interference microscopy
(090.1995) Holography : Digital holography

ToC Category:
Holography

History
Original Manuscript: January 19, 2012
Revised Manuscript: March 21, 2012
Manuscript Accepted: March 24, 2012
Published: May 30, 2012

Virtual Issues
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Natan T. Shaked, "Quantitative phase microscopy of biological samples using a portable interferometer," Opt. Lett. 37, 2016-2018 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-37-11-2016


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