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Fluorescence holography with improved signal-to-noise ratio by near image plane recording |
Optics Letters, Vol. 37, Issue 13, pp. 2445-2447 (2012)
http://dx.doi.org/10.1364/OL.37.002445
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Abstract
Holographic fluorescence imaging is very promising, as it can obtain three-dimensional fluorescence imaging without scanning. However, the current method usually records holograms far from the image plane, with the fluorescence decaying when spreading broadly. Here we show that the signal-to-noise ratio (SNR) of fluorescence holography can be improved by recording the high-contrast interferogram near the image plane. We found that this can be achieved by setting the focal length of the lens for the reference wave (
© 2012 Optical Society of America
OCIS Codes
(090.2880) Holography : Holographic interferometry
(110.6880) Imaging systems : Three-dimensional image acquisition
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(090.1995) Holography : Digital holography
ToC Category:
Holography
History
Original Manuscript: March 12, 2012
Revised Manuscript: April 9, 2012
Manuscript Accepted: April 19, 2012
Published: June 18, 2012
Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics
Citation
Xiaomin Lai, Yuan Zhao, Xiaohua Lv, Zhenqiao Zhou, and Shaoqun Zeng, "Fluorescence holography with improved signal-to-noise ratio by near image plane recording," Opt. Lett. 37, 2445-2447 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-37-13-2445
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