OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 9 — Aug. 28, 2012

Ultrafast, large-field multiphoton microscopy based on an acousto-optic deflector and a spatial light modulator

Yonghong Shao, Wan Qin, Honghai Liu, Junle Qu, Xiang Peng, Hanben Niu, and Bruce Z. Gao  »View Author Affiliations


Optics Letters, Vol. 37, Issue 13, pp. 2532-2534 (2012)
http://dx.doi.org/10.1364/OL.37.002532


View Full Text Article

Enhanced HTML    Acrobat PDF (237 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We present an ultrafast, large-field multiphoton excitation fluorescence microscope with high lateral and axial resolutions based on a two-dimensional (2-D) acousto-optical deflector (AOD) scanner and spatial light modulator (SLM). When a phase-only SLM is used to shape the near-infrared light from a mode-locked titanium:sapphire laser into a multifocus array including the 0-order beam, a 136μm×136μm field of view is achieved with a 60× objective using a 2-D AOD scanner without any mechanical scan element. The two-photon fluorescence image of a neuronal network that was obtained using this system demonstrates that our microscopy permits observation of dynamic biological events in a large field with high-temporal and -spatial resolution.

© 2012 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.7160) Medical optics and biotechnology : Ultrafast technology
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Microscopy

History
Original Manuscript: March 6, 2012
Revised Manuscript: April 29, 2012
Manuscript Accepted: May 9, 2012
Published: June 21, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Yonghong Shao, Wan Qin, Honghai Liu, Junle Qu, Xiang Peng, Hanben Niu, and Bruce Z. Gao, "Ultrafast, large-field multiphoton microscopy based on an acousto-optic deflector and a spatial light modulator," Opt. Lett. 37, 2532-2534 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-37-13-2532


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. W. R. Zipfel, R. M. Williams, and W. W. Webb, Nat. Biotechnol. 21, 1369 (2003). [CrossRef]
  2. N. Ji, J. C. Magee, and E. Betzig, Nat. Methods 5, 197 (2008). [CrossRef]
  3. A. Vogel, J. Noack, G. Hüttman, and G. Paltauf, Appl. Phys. B 81, 1015 (2005). [CrossRef]
  4. R. D. Roorda, T. M. Hohl, R. Toledo-Crow, and G. Miesenböck, J. Neurophysiol. 92, 609 (2004). [CrossRef]
  5. S. R. Goldstein, T. Hubin, S. Rosenthal, and C. Washburn, J. Microsc. 157, 29 (1990). [CrossRef]
  6. R. Salomé, Y. Kremer, S. Dieudonné, J.-F. Léger, O. Krichevsky, C. Wyart, D. Chatenay, and L. Bourdieu, J. Neurosci. Methods 154, 161 (2006). [CrossRef]
  7. V. Iyer, Br. E. Losavio, and P. Saggau, J. Biomed. Opt. 8, 460 (2003). [CrossRef]
  8. S. Zeng, X. Lv, C. Zhan, W. R. Chen, W. Xiong, S. L. Jacques, and Q. Luo, Opt. Lett. 31, 1091 (2006). [CrossRef]
  9. B. E. Losavio, V. Iyer, S. Patel, and P. Saggau, J. Neural Eng. 7, 045002 (2010). [CrossRef]
  10. J. D. Lechleiter, D.-T. Lin, and I. Sieneart, Biophys. J. 83, 2292 (2002). [CrossRef]
  11. J. Bewersdorf, R. Pick, and S. W. Hell, Opt. Lett. 23, 655 (1998). [CrossRef]
  12. V. Andresen, A. Egner, and S. W. Hell, Opt. Lett. 26, 75 (2001). [CrossRef]
  13. V. Nikolenko, B. O. Watson, R. Araya, A. Woodruff, D. S. Peterka, and R. Yuste, Front. Neural Circuits 2, 5 (2008). [CrossRef]
  14. W. Qin, Y. Shao, H. Liu, X. Peng, H. Niu, and B. Gao, Opt. Lett. 37, 827 (2012). [CrossRef]
  15. M. Agour, E. Kolenovic, C. Falldorf, and C. von Kopylow, J. Opt. Pure Appl. Opt. 11, 105405 (2009). [CrossRef]
  16. M. J. Escuti and W. M. Jones, Proc. SPIE 6332, 63320M (2006). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1. Fig. 2. Fig. 3.
 
Fig. 4.
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited