We introduce a single-exposure widefield system of producing phase gradient images by dividing the illumination and detection apertures into two oblique complementary components and encoding them using polarization. A Wollaston prism splits the images formed by the two respective aperture halves to allow both components to be simultaneously imaged by a single camera. By producing images characteristically similar to differential interference contrast while using a darkfield illumination scheme, sensitivity to weak phase gradients is improved.
© 2012 Optical Society of America
Original Manuscript: May 30, 2012
Revised Manuscript: July 20, 2012
Manuscript Accepted: August 8, 2012
Published: September 6, 2012
Vol. 7, Iss. 11 Virtual Journal for Biomedical Optics
Kengyeh K. Chu and Jerome Mertz, "Single-exposure complementary aperture phase microscopy with polarization encoding," Opt. Lett. 37, 3798-3800 (2012)