OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 3 — Feb. 29, 2012

Toward single-molecule detection with sensors based on propagating surface plasmons

Pavel Kvasnička, Karel Chadt, Milan Vala, Markéta Bocková, and Jiří Homola  »View Author Affiliations


Optics Letters, Vol. 37, Issue 2, pp. 163-165 (2012)
http://dx.doi.org/10.1364/OL.37.000163


View Full Text Article

Enhanced HTML    Acrobat PDF (320 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Surface plasmon resonance (SPR) sensors are known to be able to detect very low surface concentrations of (bio)molecules on macroscopic areas. To explore the potential of SPR biosensors to achieve single-molecule detection, we have minimized the read-out area (to 64μm2) by employing a sensor system based on spectroscopy of surface plasmons generated on a diffractive structure via a microscope objective and light collection through a small aperture. This approach allows for decreasing the number of detected molecules by 3 orders of magnitude compared to state-of-the-art SPR sensors. A protein monolayer has been shown to produce a response of 5000 times the baseline noise, suggesting that as few as 500 proteins could be detected by the sensor.

© 2012 Optical Society of America

OCIS Codes
(230.1950) Optical devices : Diffraction gratings
(240.0310) Optics at surfaces : Thin films
(240.6680) Optics at surfaces : Surface plasmons
(240.6690) Optics at surfaces : Surface waves
(240.6700) Optics at surfaces : Surfaces

ToC Category:
Optics at Surfaces

History
Original Manuscript: October 6, 2011
Revised Manuscript: November 20, 2011
Manuscript Accepted: November 21, 2011
Published: January 10, 2012

Virtual Issues
Vol. 7, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Pavel Kvasnička, Karel Chadt, Milan Vala, Markéta Bocková, and Jiří Homola, "Toward single-molecule detection with sensors based on propagating surface plasmons," Opt. Lett. 37, 163-165 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-37-2-163


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. R. L. Rich and D. G. Myszka, J. Mol. Recognit. 23, 1 (2010). [CrossRef]
  2. J. Homola, Chem. Rev. 108, 462 (2008). [CrossRef]
  3. M. Piliarik and J. Homola, Opt. Express 17, 16505 (2009). [CrossRef]
  4. M. Piliarik and J. Homola, Sens. Actuators B 134, 353 (2008). [CrossRef]
  5. T. Sannomiya and J. Voros, Trends Biotechnol. 29, 343 (2011). [CrossRef]
  6. A. D. McFarland and R. P. Van Duyne, Nano Lett. 3, 1057 (2003). [CrossRef]
  7. K. M. Mayer, F. Hao, S. Lee, P. Nordlander, and J. H. Hafner, Nanotechnology 21, 255503 (2010). [CrossRef]
  8. X. D. Su, Y. J. Wu, R. Robelek, and W. Knoll, Langmuir 21, 348 (2005). [CrossRef]
  9. K. Pimková, M. Bocková, K. Hegnerová, J. Suttnar, J. Čermák, J. Homola, and J. Dyr, “Surface plasmon resonance biosensor for the detection of VEGFR-1—a protein marker of myelodysplastic syndromes,” Anal. Bioanal. Chem., doi:10.1007/s00216-011-5395-3 (to be published). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1. Fig. 2. Fig. 3.
 
Fig. 4.
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited