OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 6 — Jun. 13, 2006

Task-based imaging of colon cancer in the ApcMin∕+ mouse model

James B. McNally, Nathaniel D. Kirkpatrick, Lida P. Hariri, Alexandre R. Tumlinson, David G. Besselsen, Eugene W. Gerner, Urs Utzinger, and Jennifer K. Barton  »View Author Affiliations


Applied Optics, Vol. 45, Issue 13, pp. 3049-3062 (2006)
http://dx.doi.org/10.1364/AO.45.003049


View Full Text Article

Enhanced HTML    Acrobat PDF (2367 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Optical coherence tomography (OCT), laser-induced fluorescence (LIF), and laser-scanning confocal microscopy (LSCM) were used for the task of multimodal study of healthy and adenomatous mouse colon. The results from each modality were compared with histology, which served as the gold standard. The A p c M i n / + genetic mouse model of colon cancer was compared with wild-type mice. In addition, a special diet was used for the task of studying the origins of a 680   nm autofluorescent signal that was previously observed in colon. The study found close agreement among each of the modalities and with histology. All four modalities were capable of identifying diseased tissue accurately. The OCT and LSCM images provided complementary structural information about the tissue, while the autofluorescence signal measured by LIF and LSCM provided biochemical information. OCT and LIF were performed in vivo and nondestructively, while the LSCM and histology required extraction of the tissue. The magnitude of the 680   nm signal correlates with chlorophyll content in the mouse diet, suggesting that the autofluorescent compound is a dietary metabolite.

© 2006 Optical Society of America

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.2680) Medical optics and biotechnology : Gastrointestinal
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

ToC Category:
Application

History
Original Manuscript: May 16, 2005
Revised Manuscript: December 4, 2005
Manuscript Accepted: December 9, 2005

Virtual Issues
Vol. 1, Iss. 6 Virtual Journal for Biomedical Optics

Citation
James B. McNally, Nathaniel D. Kirkpatrick, Lida P. Hariri, Alexandre R. Tumlinson, David G. Besselsen, Eugene W. Gerner, Urs Utzinger, and Jennifer K. Barton, "Task-based imaging of colon cancer in the ApcMin/+ mouse model," Appl. Opt. 45, 3049-3062 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-45-13-3049

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited