OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 6 — Jun. 13, 2006

Airborne bacterial endospores detected by use of an impinger containing aqueous terbium chloride

David L. Rosen  »View Author Affiliations

Applied Optics, Vol. 45, Issue 13, pp. 3152-3157 (2006)

View Full Text Article

Enhanced HTML    Acrobat PDF (626 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



An experimental investigation is described of a terbium luminescence sensor for continuous collection and detection of bacterial endospores suspended in the atmosphere. A sensor is presented that continuously monitors and rapidly detects small concentrations of aerosolized bacterial endospores. The aerosol particles are collected by an impinger from which terbium dipicolinate photoluminescence is measured. A preliminary test of the sensor is described with Bacillus globigii endospores that were dispersed in an aerosol chamber. Another experiment is described showing that evaporation of water from the impinger limits operation time of the sensor to a few hours.

© 2006 Optical Society of America

OCIS Codes
(010.1110) Atmospheric and oceanic optics : Aerosols
(120.1880) Instrumentation, measurement, and metrology : Detection
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence
(300.6500) Spectroscopy : Spectroscopy, time-resolved

ToC Category:
Atmospheric and Oceanic Optics

Original Manuscript: July 20, 2005
Manuscript Accepted: September 16, 2005

Virtual Issues
Vol. 1, Iss. 6 Virtual Journal for Biomedical Optics

David L. Rosen, "Airborne bacterial endospores detected by use of an impinger containing aqueous terbium chloride," Appl. Opt. 45, 3152-3157 (2006)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. M. P. Buttner, K. Willeke, and S. A. Grinshpun, "Sampling and Analysis of Airborne Microorganisms," Manual of Environmental Microbiology (ASM Press, 1997), pp. 629-640.
  2. S. A. Grinshpun, K. Willeke, V. Ulevicius, A. Juozaitis, S. Terzieva, J. Donnelly, G. N. Stelma, and K. P. Brenner, "Effect of Impaction, Bounce and Reaerosolization on the Collection Efficiency of Impingers," Aerosol Sci. Technol. 26, 326-342 (1997). [CrossRef]
  3. O. Pahl, V. R. Phillips, J. Lacey, J. Hartung, and C. M. Wathes, "Comparison of commonly used samplers with a novel bioaerosol sampler with automatic plate exchange," J. Aerosol Sci. 28, 427-435 (1997). [CrossRef]
  4. C. S. Li, M. L. Hao, C. W. Chang, and C. S. Wang, "Evaluation of Microbial Samplers for Bacterial Microorganisms," Aerosol Sci. Technol. 30, 100-108 (1999). [CrossRef]
  5. P. P. Hairston, J. Ho, and F. R. Quant, "Design of an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence," J. Aerosol. Sci. 28, 471-482 (1997). [CrossRef] [PubMed]
  6. D. L. Rosen, "Bacterial endospore detection using photoluminescence from terbium dipicolinate," Rev. Anal. Chem. 18, 1-21 (1999). [CrossRef]
  7. D. L. Rosen and S. Niles, "Chelation number of terbium dipicolinate: Effects on photoluminescence lifetime and absorption," Appl. Spectrosc. 55, 208-216 (2001). [CrossRef]
  8. A. A. Hindle and E. A. H. Hall, "Dipicolinic acid (DPA) assay revisited and appraised for spore detection," Analyst 124, 1599-1604 (1999). [CrossRef]
  9. P. A. Jensen, W. F. Todd, G. N. Davis, and P. V. Scarpino, "Evaluation of eight bioaerosol samplers challenged with aerosols of free bacteria," Am. Ind. Hyg. Assoc. J. 53, 660-667 (1992). [CrossRef] [PubMed]
  10. K. Willeke, X. Lin, and S. A. Grinshpun, "Improved aerosol collection by combined impaction and centrifugal motion," Aerosol Sci. Technol. 28, 439-456 (1998). [CrossRef]
  11. E. D. Lester and A. Ponce, "An anthrax smoke detector: Online detection of aerosolized bacterial spores," IEEE Eng. Med. Biol. Mag. 21, 38-42 (2002). [CrossRef] [PubMed]
  12. E. D. Lester, G. Bearman, and A. Ponce, "A second-generation anthrax smoke detector: an inexpensive front-end monitor that detects airborne bacterial spores," IEEE Eng. Med. Biol. Mag. 23, 130-135 (2004). [CrossRef] [PubMed]
  13. G. Jones and V. I. Vulev, "Medium effects on the photophysical properties of terbium (III) complexes with pyridine-2, 6 dicarboxylate," Photochem. Photobiol. Sci. 1, 925-933 (2002). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited