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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 7 — Jul. 17, 2006

Optical trapping of a spherically symmetric sphere in the ray-optics regime: a model for optical tweezers upon cells

Yi-Ren Chang, Long Hsu, and Sien Chi  »View Author Affiliations


Applied Optics, Vol. 45, Issue 16, pp. 3885-3892 (2006)
http://dx.doi.org/10.1364/AO.45.003885


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Abstract

Since their invention in 1986, optical tweezers have become a popular manipulation and force measurement tool in cellular and molecular biology. However, until recently there has not been a sophisticated model for optical tweezers on trapping cells in the ray-optics regime. We present a model for optical tweezers to calculate the optical force upon a spherically symmetric multilayer sphere representing a common biological cell. A numerical simulation of this model shows that not only is the magnitude of the optical force upon a Chinese hamster ovary cell significantly three times smaller than that upon a polystyrene bead of the same size, but the distribution of the optical force upon a cell is also much different from that upon a uniform particle, and there is a 30% difference in the optical trapping stiffness of these two cases. Furthermore, under a small variant condition for the refractive indices of any adjacent layers of the sphere, this model provides a simple approximation to calculate the optical force and the stiffness of an optical tweezers system.

© 2006 Optical Society of America

OCIS Codes
(080.2720) Geometric optics : Mathematical methods (general)
(140.7010) Lasers and laser optics : Laser trapping
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation

History
Original Manuscript: April 6, 2005
Revised Manuscript: July 29, 2005
Manuscript Accepted: July 29, 2005

Virtual Issues
Vol. 1, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Yi-Ren Chang, Long Hsu, and Sien Chi, "Optical trapping of a spherically symmetric sphere in the ray-optics regime: a model for optical tweezers upon cells," Appl. Opt. 45, 3885-3892 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-45-16-3885


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