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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 7 — Jul. 17, 2006

Steady-state directional diffuse reflectance and fluorescence of human skin

Kamal M. Katika and Laurent Pilon  »View Author Affiliations


Applied Optics, Vol. 45, Issue 17, pp. 4174-4183 (2006)
http://dx.doi.org/10.1364/AO.45.004174


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Abstract

We present numerical simulations predicting the directional diffuse reflectance and autofluorescence from human skin. Skin is modeled as a seven-layered medium, with each layer having its own optical properties and fluorophore concentrations. Both collimated and diffuse monochromatic excitation at 442 nm are considered. In addition, the effect of an index-matching cream used to eliminate total internal reflection within the skin is assessed. We compute the intensity distributions of the excitation and fluorescence light in the skin by solving the radiative transfer equation using the modified method of characteristics. It was found that the use of an index-matching cream reduces the directional fluorescence signal while increasing the directional diffuse reflectance from the skin for collimated excitation. On the other hand, both the fluorescence and diffuse reflectance increase for diffuse excitation with an index-matching cream. Moreover, the directional fluorescence intensity obtained by use of collimated excitation is larger than that obtained by use of diffuse excitation light. This computational tool could be valuable in designing optical devices for biomedical applications.

© 2006 Optical Society of America

OCIS Codes
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.7050) Medical optics and biotechnology : Turbid media
(290.7050) Scattering : Turbid media
(300.2530) Spectroscopy : Fluorescence, laser-induced

History
Original Manuscript: May 9, 2005
Revised Manuscript: September 12, 2005
Manuscript Accepted: January 5, 2006

Virtual Issues
Vol. 1, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Kamal M. Katika and Laurent Pilon, "Steady-state directional diffuse reflectance and fluorescence of human skin," Appl. Opt. 45, 4174-4183 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-45-17-4174


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References

  1. J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Kluwer Academic/Plenum, 1999).
  2. R. Richards-Kortum and E. Sevick-Muraca, "Quantitative optical spectroscopy for tissue diagnostics," Annu. Rev. Phys. Chem. 47, 555-606 (1996). [CrossRef]
  3. K. Katika and L. Pilon, "Modified method of characteristics in transient radiative transfer," J. Quant. Spectrosc. Radiat. Transfer 98, 220-237 (2006). [CrossRef]
  4. H. Zeng and C. MacAulay, "Fluorescence spectroscopy and imaging for skin cancer detection and evaluation," in Handbook of Biomedical Fluorescence (Marcel Dekker, 2003), pp. 315-360.
  5. M. J. C. V. Gemert, S. L. Jacques, H. J. C. M. Sterenborg, and W. M. Star, "Skin optics," IEEE Trans. Biomed. Eng. 36, 1146-1154 (1989). [CrossRef]
  6. N. Kollias, G. Zonios, and G. N. Stamatas, "Fluorescence spectroscopy of skin," Vib. Spectrosc. 28, 17-23 (2002). [CrossRef]
  7. K. Koenig, H. Schneckenburger, J. Hemmer, B. J. Tromberg, and R. W. Steiner, "In-vivo fluorescence detection and imaging of porphyrin-producing bacteria in the human skin and in the oral cavity for diagnosis of acne vulgaris, caries, and squamous cell carcinoma," in Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases, R. R. Alfano, ed., Proc. SPIE 2135, 129-138 (1994). [CrossRef]
  8. Y. Takema, Y. Yorimoto, H. Ohsu, O. Osanai, and M. Kawai, "Age-related discontinuous changes in the in vivo fluorescence of human facial skin," J. Dermatolog. Sci. 24, 55-58 (1997). [CrossRef]
  9. N. Kollias, R. Gillies, M. Moran, I. E. Kochevar, and R. R. Anderson, "Endogenous skin fluorescence includes bands that may serve as quantitative markers of aging and photoaging," J. Invest. Dermatol. 111, 776-776 (1998). [CrossRef]
  10. R. H. Na, I. M. Stender, M. Henriksen, and H. C. Wulf, "Autofluorescence of human skin is age-related after correction for skin pigmentation and redness," J. Invest. Dermatol. 116, 536-540 (2001). [CrossRef]
  11. J. Sandby-Moller, E. Thieden, P. A. Philipsen, J. Heydenreich, and H. C. Wulf, "Skin autofluorescence as a biological UVR dosimeter," Photodermatol. Photoimmunol. Photomed. 20, 33-40 (2004). [CrossRef]
  12. R. Gillies, G. Zonios, R. R. Anderson, and N. Kollias, "Fluorescence excitation spectroscopy provides information about human skin in vivo," J. Invest. Dermatology 115, 704-707 (2000). [CrossRef]
  13. L. Brancaleon, A. J. Durkin, J. H. Tu, G. Menaker, J. D. Fallon, and N. Kollias, "In vivo fluorescence spectroscopy of nonmelanoma skin cancer," Photochem. Photobiol. 73, 178-183 (2001). [CrossRef]
  14. H. Zeng, D. I. McLean, C. E. MacAulay, B. Palcic, and H. Lui, "Autofluorescence of basal cell carcinoma," in Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems VIII, R. R. Anderson, K. E. Bartels, L. S. Bass, C. G. Garrett, K. W. Gregory, H. Lui, R. S. Malek, A. P. Perlmutter, L. Reinisch, P. J. Smalley, L. P. Tate, S. L. Thomsen, and G. M. Watson, eds., Proc. SPIE 3245, 314-317 (1998). [CrossRef]
  15. H. Zeng, D. I. McLean, C. E. MacAulay, and H. Lui, "Autofluorescence properties of skin and applications in dermatology," in Biomedical Photonics and Optoelectronic Imaging, H. Liu and Q. Luo, eds., Proc. SPIE 4224, 366-373 (2000). [CrossRef]
  16. M. Panjehpour, C. E. Julius, M. N. Phan, T. Vo-Dinh, and B. F. Overholt, "In-vivo fluorescence spectroscopy for diagnosis of skin cancer," in Biomedical Diagnostic, Guidance, and Surgical-Assist Systems IV, T. Vo-Dinh, D. A. Benaron, and W. S. Grundfest, eds., Proc. SPIE 4615, 20-31 (2002). [CrossRef]
  17. T. Vo-Dinh, M. Panjehpour, B. F. Overholt, C. E. Julius, S. Overholt, and M. N. Phan, "Laser-induced fluorescence for the detection of esophageal and skin cancer," in Advanced Biomedical and Clinical Diagnostic Systems, T. Vo-Dinh, W. S. Grundfest, D. A. Benaron, and G. E. Cohn, eds., Proc. SPIE 4958, 67-70 (2003). [CrossRef]
  18. R. Meerwaldt, R. Graaff, P. H. N. Oomen, T. P. Links, J. J. Jager, N. L. Alderson, S. R. Thorpe, J. W. Baynes, R. O. B. Gans, and A. J. Smit, "Simple non-invasive assessment of advanced glycation endproduct accumulation," Diabetologia 47, 1324-1330 (2004). [CrossRef]
  19. Y. P. Sinichkin, S. R. Utz, and H. A. Pilipenko, "Laser-induced fluorescence of the human skin in vivo: influence of the erythema," in Optical Biopsy, R. Cubeddu, S. Svanberg, and H. van den Bergh, eds., Proc. SPIE 2081, 41-47 (1994). [CrossRef]
  20. Y. P. Sinichkin, S. R. Utz, P. M. Yudin, and H. A. Pilipenko, "Investigation of formation and dynamics of human skin erythema and pigmentation by in vivo fluorescence spectroscopy," in Optical Biopsy and Fluorescence Spectroscopy and Imaging, R. Cubeddu, R. Marchesini, S. R. Mordon, K. Svanberg, H. H. Rinneberg, and G. A. Wagnieres, eds., Proc. SPIE 2324, 259-268 (1995). [CrossRef]
  21. S. R. Utz, Y. P. Sinichkin, I. V. Meglinski, and H. A. Pilipenko, "Fluorescence spectroscopy in combination with reflectance measurements in human skin examination: what for and how," in Optical Biopsy and Fluorescence Spectroscopy and Imaging, R. Cubeddu, R. Marchesini, S. R. Mordon, K. Svanberg, H. H. Rinneberg, and G. A. Wagnieres, eds., Proc. SPIE 2324, 125-136 (1995). [CrossRef]
  22. H. Zeng, C. E. MacAulay, B. Palcic, and D. I. McLean, "Laser-induced changes in autofluorescence of in-vivo skin," in Laser-Tissue Interaction IV, S. L. Jacques and A. Katzir, eds., Proc. SPIE 1882, 278-290 (1993). [CrossRef]
  23. H. Zeng, C. MacAulay, D. I. McLean, and B. Palcic, "Spectroscopic and microscopic characteristics of human skin autofluorescence emission," Photochem. Photobiol. 61, 639-645 (1995).
  24. H. Zeng, C. E. MacAulay, D. I. McLean, and B. Palcic, "Spectroscopy and microscopy studies of skin tissue autofluorescence emission," in Optical Biopsy and Fluorescence Spectroscopy and Imaging, R. Cubeddu, R. Marchesini, S. R. Mordon, K. Svanberg, H. H. Rinneberg, and G. A. Wagnieres, eds., Proc. SPIE 2324, 198-207 (1995). [CrossRef]
  25. H. Zeng, H. Lui, D. I. McLean, C. E. MacAulay, and B. Palcic, "Optical spectroscopy studies of diseased skin: preliminary results," in Optical and Imaging Techniques for Biomonitoring, H.-J. Foth, R. Marchesini, H. Podbielska, M. R. Nicoud, and H. Schneckenburger, eds., Proc. SPIE 2628, 281-285 (1996).
  26. H. Zeng, C. E. MacAulay, B. Palcic, and D. I. McLean, "Monte Carlo modeling of tissue autofluorescence measurement and imaging," in Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases, R. R. Alfano, ed., Proc. SPIE 2135, 94-104 (1994). [CrossRef]
  27. H. Zeng, C. MacAulay, D. I. McLean, and B. Palcic, "Reconstruction of in vivo skin autofluorescence spectrum from microscopic properties by Monte Carlo simulation," J. Photochem. Photobiol. B 38, 234-240 (1997). [CrossRef]
  28. H. Zeng, C. E. MacAulay, B. Palcic, and D. I. McLean, "Autofluorescence distribution in skin tissue revealed by microspectrophotometer measurements," in Lasers in Otolaryngology, Dermatology, and Tissue Welding, R. R. Anderson, L. S. Bass, S. M. Shapshay, J. V. White, and R. A. White, eds., Proc. SPIE 1876, 129-135 (1993). [CrossRef]
  29. M. Keijzer, R. Richards-Kortum, S. Jacques, and M. Feld, "Fluorescence spectroscopy of turbid media: autofluorescence of the human aorta," Appl. Opt. 28, 4286-4292 (1989).
  30. I. V. Meglinski and D. Y. Churmakov, "A novel Monte Carlo method for the optical diagnostics of skin," in Diagnostic Optical Spectroscopy in Biomedicine II, G. A. Wagnieres, ed., Proc. SPIE 5141, 133-141 (2003). [CrossRef]
  31. I. V. Meglinski, "Monte Carlo method in optical diagnostics of skin and skin tissues," in Third International Conference on Photonics and Imaging in Biology and Medicine, Q. Luo, V. V. Tuchin, M. Gu, and L. V. Wang, eds., Proc. SPIE 5254, 30-43 (2003). [CrossRef]
  32. M. F. Modest, Radiative Heat Transfer (Academic, 2002).
  33. D. Y. Churmakov, I. V. Meglinski, S. A. Piletsky, and D. A. Greenhalgh, "Skin fluorescence model based on the Monte Carlo technique," in Saratov Fall Meeting 2002: Optical Technologies in Biophysics and Medicine IV, V. V. Tuchin, ed., Proc. SPIE 5068, 326-333 (2003). [CrossRef]
  34. D. Y. Churmakov, I. V. Meglinski, and D. A. Greenhalgh, "Amending of fluorescence sensor signal localization in human skin by matching of the refractive index," J. Biomed. Opt. 9, 339-346 (2004). [CrossRef]
  35. D. Y. Churmakov, I. V. Meglinski, and D. A. Greenhalgh, "Automatic amending of the tattoo sensor fluorescence localization by refractive index matching," in Diagnostic Optical Spectroscopy in Biomedicine II, G. A. Wagnieres, ed., Proc. SPIE 5141, 122-132 (2003). [CrossRef]
  36. D. Y. Churmakov, I. V. Meglinski, S. A. Piletsky, and D. A. Greenhalgh, "Automatic enhancement of skin fluorescence localization due to refractive index matching," in ALT'03 International Conference on Advanced Laser Technologies: Bio-medical Optics, R. K. Wang, J. C. Hebden, A. V. Priezzhev, and V. V. Tuchin, eds., Proc. SPIE 5486, 16-27 (2004). [CrossRef]
  37. A. Ishimaru, "Diffusion of light in turbid material," Appl. Opt. 28, 2210-2215 (1989).
  38. T. J. Farrell and M. S. Patterson, "Diffusion modeling of fluorescence in tissue," in Handbook of Biomedical Fluorescence (Marcel Dekker, 2003), pp. 29-60.
  39. A. H. Hielscher, R. E. Alcouffe, and R. L. Barbour, "Comparison of finite-difference transport and diffusion calculations for photon migration in homogeneous and heterogeneous tissues," Phys. Med. Biol. 43, 1285-1302 (1998). [CrossRef]
  40. T. Spott and L. O. Svaasand, "Collimated light sources in the diffusion approximation," Appl. Opt. 39, 6453-6465 (2000).
  41. G. Yoon, S. A. Prahl, and A. J. Welch, "Accuracies of the diffusion approximation and its similarity relations for laser irradiated biological media," Appl. Opt. 28, 2250-2255 (1989).
  42. L.-H. Wang andS. L. Jacques, Monte Carlo Modeling of Light Transport in Multi-Layered Tissues in Standard C (University of Texas M. D. Anderson Cancer Center, 1992).
  43. J. Q. Lu, X.-H. Hu, and K. Dong, "Modeling of the rough-interface effect on a converging light beam propagating in a skin tissue phantom," Appl. Opt. 39, 5890-5897 (2000).
  44. I. V. Meglinski and S. J. Matcher, "Modeling of skin reflectance spectra," in Saratov Fall Meeting 2000: Optical Technologies in Biophysics and Medicine II, V. V. Tuchin, ed., Proc. SPIE 4241, 78-87 (2001). [CrossRef]

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