We simulate and measure light scattering of a micrometer-sized spherical particle suspended in solution close to a glass substrate. The model, based on the discrete sources method, is developed to describe the experimental situation of total internal reflection microscopy experiments; i.e., the particle is illuminated by an evanescent light field originating from the glass–solvent interface. In contrast to the well-established assumption of a simple exponential decay of the scattering intensity with distance, we demonstrate significant deviations for a certain range of penetration depths and polarization states of the incident light.
© 2006 Optical Society of America
Optics at Surfaces
Original Manuscript: March 8, 2006
Revised Manuscript: June 14, 2006
Manuscript Accepted: June 15, 2006
Vol. 1, Iss. 11 Virtual Journal for Biomedical Optics
Laurent Helden, Elena Eremina, Norbert Riefler, Christopher Hertlein, Clemens Bechinger, Yuri Eremin, and Thomas Wriedt, "Single-particle evanescent light scattering simulations for total internal reflection microscopy," Appl. Opt. 45, 7299-7308 (2006)