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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 11 — Nov. 13, 2006

Single-particle evanescent light scattering simulations for total internal reflection microscopy

Laurent Helden, Elena Eremina, Norbert Riefler, Christopher Hertlein, Clemens Bechinger, Yuri Eremin, and Thomas Wriedt  »View Author Affiliations


Applied Optics, Vol. 45, Issue 28, pp. 7299-7308 (2006)
http://dx.doi.org/10.1364/AO.45.007299


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Abstract

We simulate and measure light scattering of a micrometer-sized spherical particle suspended in solution close to a glass substrate. The model, based on the discrete sources method, is developed to describe the experimental situation of total internal reflection microscopy experiments; i.e., the particle is illuminated by an evanescent light field originating from the glass–solvent interface. In contrast to the well-established assumption of a simple exponential decay of the scattering intensity with distance, we demonstrate significant deviations for a certain range of penetration depths and polarization states of the incident light.

© 2006 Optical Society of America

OCIS Codes
(120.5820) Instrumentation, measurement, and metrology : Scattering measurements
(240.0240) Optics at surfaces : Optics at surfaces
(290.4020) Scattering : Mie theory

ToC Category:
Optics at Surfaces

History
Original Manuscript: March 8, 2006
Revised Manuscript: June 14, 2006
Manuscript Accepted: June 15, 2006

Virtual Issues
Vol. 1, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Laurent Helden, Elena Eremina, Norbert Riefler, Christopher Hertlein, Clemens Bechinger, Yuri Eremin, and Thomas Wriedt, "Single-particle evanescent light scattering simulations for total internal reflection microscopy," Appl. Opt. 45, 7299-7308 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-45-28-7299

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