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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 11 — Nov. 13, 2006

Fiber-optic fluorescence correlation spectrometer

Kanchan Garai, Mohan Muralidhar, and Sudipta Maiti  »View Author Affiliations


Applied Optics, Vol. 45, Issue 28, pp. 7538-7542 (2006)
http://dx.doi.org/10.1364/AO.45.007538


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Abstract

Fluorescence correlation spectroscopy (FCS) is a sensitive technique used to probe size, concentration, flow velocity, and reaction kinetics in a dilute solution. Conventional FCS spectrometers achieve this sensitivity at the cost of using bulky optics. We demonstrate a technique that utilizes a single-mode optical fiber of 3.3 μ m mode field diameter to perform FCS measurements. We demonstrate that the technique has adequate sensitivity to perform FCS measurements on fluorescent beads of 13   nm radius, and that the results agree with theoretical predictions. Our method potentially allows FCS to be extended to remote and in vivo applications.

© 2006 Optical Society of America

OCIS Codes
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: December 14, 2005
Revised Manuscript: March 16, 2006
Manuscript Accepted: May 18, 2006

Virtual Issues
Vol. 1, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Kanchan Garai, Mohan Muralidhar, and Sudipta Maiti, "Fiber-optic fluorescence correlation spectrometer," Appl. Opt. 45, 7538-7542 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-45-28-7538


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