We report bright-field and dark-field surface-plasmon imaging using a modified solid immersion lens and a commercial objective of moderate NA in the epi configuration. The contrast and resolution are extremely good, giving well-resolved images of protein monolayers both in air and in water. We also describe a two-part solid immersion lens that allows the sample to be moved without degrading the image quality in any observable way. The merits of the two-part lens are discussed and compared to commercially available microscope objectives. Finally, we introduce a simple Green's function model that illustrates the key features of both bright-field and dark-field surface-plasmon imaging.
© 2006 Optical Society of America
Original Manuscript: March 22, 2006
Manuscript Accepted: May 18, 2006
Vol. 1, Iss. 12 Virtual Journal for Biomedical Optics
Jing Zhang, Mark C. Pitter, Shugang Liu, Chungwah See, and Mike G. Somekh, "Surface-plasmon microscopy with a two-piece solid immersion lens: bright and dark fields," Appl. Opt. 45, 7977-7986 (2006)