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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 12 — Dec. 18, 2006

Submicrometer optical tomography by multiple-wavelength digital holographic microscopy

Frédéric Montfort, Tristan Colomb, Florian Charrière, Jonas Kühn, Pierre Marquet, Etienne Cuche, Sylvain Herminjard, and Christian Depeursinge  »View Author Affiliations


Applied Optics, Vol. 45, Issue 32, pp. 8209-8217 (2006)
http://dx.doi.org/10.1364/AO.45.008209


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Abstract

We present a method for submicrometer tomographic imaging using multiple wavelengths in digital holographic microscopy. This method is based on the recording, at different wavelengths equally separated in the k domain, in off-axis geometry, of the interference between a reference wave and an object wave reflected by a microscopic specimen and magnified by a microscope objective. A CCD camera records the holograms consecutively, which are then numerically reconstructed following the convolution formulation to obtain each corresponding complex object wavefront. Their relative phases are adjusted to be equal in a given plane of interest and the resulting complex wavefronts are summed. The result of this operation is a constructive addition of complex waves in the selected plane and destructive addition in the others. Tomography is thus obtained by the attenuation of the amplitude out of the plane of interest. Numerical variation of the plane of interest enables one to scan the object in depth. For the presented simulations and experiments, 20 wavelengths are used in the 480 700   nm range. The result is a sectioning of the object in slices 725   nm thick.

© 2006 Optical Society of America

OCIS Codes
(090.1760) Holography : Computer holography
(100.3010) Image processing : Image reconstruction techniques
(110.0180) Imaging systems : Microscopy
(110.6880) Imaging systems : Three-dimensional image acquisition
(110.6960) Imaging systems : Tomography

ToC Category:
Imaging Systems

History
Original Manuscript: June 12, 2006
Revised Manuscript: July 11, 2006
Manuscript Accepted: July 11, 2006

Virtual Issues
Vol. 1, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Frédéric Montfort, Tristan Colomb, Florian Charrière, Jonas Kühn, Pierre Marquet, Etienne Cuche, Sylvain Herminjard, and Christian Depeursinge, "Submicrometer optical tomography by multiple-wavelength digital holographic microscopy," Appl. Opt. 45, 8209-8217 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-45-32-8209


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