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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 12 — Dec. 18, 2006

Scanning transmission microscopy using a position-sensitive detector

Mark R. Ayres and Robert R. McLeod  »View Author Affiliations


Applied Optics, Vol. 45, Issue 33, pp. 8410-8418 (2006)
http://dx.doi.org/10.1364/AO.45.008410


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Abstract

Optical data manipulation technologies increasingly employ densely aperiodic optical 3D phase elements. Refinement of such technologies will require the capability to quantitatively characterize the volumetric dielectric modulation of an optical sample to a high level of precision and spatial resolution. We present a scanning transmission microscopy system that uses a position-sensitive detector to impart sensitivity to both the phase and absorption components. We describe the layout of the instrument and then derive its phase and absorption transfer functions. Simulations and experiments are presented to validate the analysis. For phase detection, the instrument possesses depth-sectioning properties similar to those of a confocal microscope without the use of a pinhole, enabling full 3D object reconstruction.

© 2006 Optical Society of America

OCIS Codes
(110.4850) Imaging systems : Optical transfer functions
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy

History
Original Manuscript: April 10, 2006
Manuscript Accepted: May 25, 2006

Virtual Issues
Vol. 1, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Mark R. Ayres and Robert R. McLeod, "Scanning transmission microscopy using a position-sensitive detector," Appl. Opt. 45, 8410-8418 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-45-33-8410

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