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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 12 — Dec. 18, 2006

Scanning transmission microscopy using a position-sensitive detector

Mark R. Ayres and Robert R. McLeod  »View Author Affiliations

Applied Optics, Vol. 45, Issue 33, pp. 8410-8418 (2006)

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Optical data manipulation technologies increasingly employ densely aperiodic optical 3D phase elements. Refinement of such technologies will require the capability to quantitatively characterize the volumetric dielectric modulation of an optical sample to a high level of precision and spatial resolution. We present a scanning transmission microscopy system that uses a position-sensitive detector to impart sensitivity to both the phase and absorption components. We describe the layout of the instrument and then derive its phase and absorption transfer functions. Simulations and experiments are presented to validate the analysis. For phase detection, the instrument possesses depth-sectioning properties similar to those of a confocal microscope without the use of a pinhole, enabling full 3D object reconstruction.

© 2006 Optical Society of America

OCIS Codes
(110.4850) Imaging systems : Optical transfer functions
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy

Original Manuscript: April 10, 2006
Manuscript Accepted: May 25, 2006

Virtual Issues
Vol. 1, Iss. 12 Virtual Journal for Biomedical Optics

Mark R. Ayres and Robert R. McLeod, "Scanning transmission microscopy using a position-sensitive detector," Appl. Opt. 45, 8410-8418 (2006)

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  1. K. Curtis, W. L. Wilson, and L. Dhar, "High density holographic storage," presented at the International Symposium on Optical Memory, Jeju Island, South Korea, 11-15 October 2004; www.isom.jp.
  2. R. R. McLeod, A. J. Daiber, M. E. McDonald, T. L. Robertson, T. Slagle, S. L. Sochava, and L. Hesselink, "Microholographic multilayer optical disk data storage," Appl. Opt. 44, 3197-3207 (2005). [CrossRef] [PubMed]
  3. R. R. McLeod, A. C. Sullivan, M. W. Grabowski, and T. F. Scott, "Hybrid integrated optics in volume holographic photopolymer," in Organic Holographic Materials and Applications II, K. Meerholz, ed., Proc SPIE 5521, 55-62 (2004).
  4. R. R. McLeod, A. C. Sullivan, and M. W. Grabowski, "Direct-write waveguides in volume photopolymers," presented at Integrated Photonics Research, San Francisco, Calif., 30 June-2 July 2004 (Optical Society of America, 2004).
  5. M. Will, S. Nolte, B. N. Chichkov, and A. Tunnermann, "Optical properties of waveguides fabricated in fused silica by femtosecond laser pulses," Appl. Opt. 41, 4360-4364 (2002). [CrossRef] [PubMed]
  6. M. Minsky, "Microscopy apparatus," U.S. Patent 3,013,467 (19 December 1961).
  7. B. R. Frieden, "Optical transfer of the three-dimensional object," J. Opt. Soc. Am. 57, 55-57 (1967). [CrossRef]
  8. N. Streibl, "Three-dimensional imaging by a microscope," J. Opt. Soc. Am. A 2, 121-127 (1985). [CrossRef]
  9. C. J. R. Sheppard and X. Q. Mao, "Three-dimensional imaging in a microscope," J. Opt. Soc. Am. A 6, 1260-1269 (1989). [CrossRef]
  10. Y. Kawata, R. Juskaitis, T. Tanaka, T. Wilson, and S. Kawata, "Differential phase-contrast microscope with a split detector for the readout system of a multilayered optical memory," Appl. Opt. 35, 2466-2470 (1996). [CrossRef] [PubMed]
  11. M. R. Ayres, "Methods for measuring optical characteristics by differential diffractive scanning," U.S. patent application 10/800,111 (27 January 2005).
  12. J. T. Wallmark, "A new semiconductor photocell using lateral photoeffect," Proc. IRE 45, 474-483 (1957). [CrossRef]
  13. H. Kogelnik, "Coupled wave theory for thick hologram gratings," Bell Syst. Tech. J. 48, 2909-2947 (1969).
  14. C. J. R. Sheppard and M. Gu, "Three-dimensional transfer functions for high-aperture systems," J. Opt. Soc. Am. A 11, 593-598 (1994). [CrossRef]
  15. C. J. R. Sheppard and A. Choudhury, "Image formation in the scanning microscope," Opt. Acta 24, 1051-1073 (1977). [CrossRef]
  16. J. W. O'Byrne, P. W. Fekete, M. R. Arnison, H. Zhao, M. Serrano, D. Philp, W. Sudiarta, and C. J. Cogswell, "Adaptive optics in confocal microscopy," in Proceedings of the Second International Workshop on Adaptive Optics for Industry and Medicine, G. D. Love, ed. (World Scientific, 1999), pp. 85-90.
  17. C. J. Sheppard and T. Wilson, "Depth of field in the scanning microscope," Opt. Lett. 3, 115-117 (1978). [CrossRef] [PubMed]
  18. M. Born and E. Wolf, Principles of Optics, 6th ed. (Cambridge U. Press, 1980), p. 437.

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