OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 1 — Jan. 19, 2007

Two-photon excitation fluorescence microscopy with a high depth of field using an axicon

Pascal Dufour, Michel Piché, Yves De Koninck, and Nathalie McCarthy  »View Author Affiliations


Applied Optics, Vol. 45, Issue 36, pp. 9246-9252 (2006)
http://dx.doi.org/10.1364/AO.45.009246


View Full Text Article

Enhanced HTML    Acrobat PDF (921 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

In conventional two-photon excitation fluorescence microscopy, the numerical aperture of the objective determines the lateral resolution and the depth of field. In some situations, as with functional imaging of dynamic events distributed in live biological tissue, an improved temporal resolution is needed; as a consequence, it is imperative to use optics with a high depth of field to simultaneously image objects at different axial positions. With a conventional microscope objective, increasing the depth of field is achieved at the expense of lateral resolution. To overcome this limitation, we have incorporated an axicon in a two-photon excitation fluorescence microscopy system; measurements have shown that an axicon provides a depth of field in excess of a millimeter, while the lateral resolution is maintained at the micrometer scale. Thus axicon-based two-photon microscopy has been shown to yield a high-resolution projection image of a sample with a single 2D scan of the laser beam while maintaining the improved tissue penetration typical of two-photon microscopy.

© 2006 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy

History
Original Manuscript: February 21, 2006
Revised Manuscript: June 14, 2006
Manuscript Accepted: July 12, 2006

Virtual Issues
Vol. 2, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Pascal Dufour, Michel Piché, Yves De Koninck, and Nathalie McCarthy, "Two-photon excitation fluorescence microscopy with a high depth of field using an axicon," Appl. Opt. 45, 9246-9252 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-45-36-9246


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. R. Cossart, D. Aronov, and R. Yuste, "Attractor dynamics of network UP states in the neocortex," Nature 423, 283-288 (2003). [CrossRef] [PubMed]
  2. W. Denk, J. H. Strickler, and W. W. Webb, "Two-photon laser scanning fluorescence microscopy," Nature 248, 73-76 (1990).
  3. A. Hopt and E. Neher, "Highly nonlinear photodamage in two-photon fluorescence microscopy," Biophys. J. 80, 2029-2036 (2001). [CrossRef] [PubMed]
  4. J. Bewersdorf, R. Pick, and S. W. Hell, "Multifocal multiphoton microscopy," Opt. Lett. 23, 655-677 (1998). [CrossRef]
  5. A. H. Buist, M. Muller, J. Squier, and G. J. Brackenhoff, "Real time two-photon absorption microscopy using multi point excitation," J. Microsc. 192, 217-226 (1998). [CrossRef]
  6. D. N. Fittinghoff, P. W. Wiseman, and J. A. Squier, "Widefield multiphoton and temporally decorrelated multifocal multiphoton microscopy," Opt. Express 7, 273-279 (2000). [CrossRef] [PubMed]
  7. S. C. Tucker, W. T. Cathey, and E. R. Dowski, "Extended depth of field and aberration control for inexpensive digital microscope systems," Opt. Express 4, 467-474 (1999). [CrossRef] [PubMed]
  8. P. Potuluri, M. R. Fetterman, and D. J. Brady, "High depth of field microscopic imaging using an interferometric camera," Opt. Express 8, 624-630 (2001). [CrossRef] [PubMed]
  9. S. Fujiwara, "Optical properties of conic surfaces. I. Reflecting cone," J. Opt. Soc. Am. 52, 287-292 (1962). [CrossRef]
  10. G. Roy and R. Tremblay, "Influence of the divergence of a laser beam on the axial intensity distribution of an axicon," Opt. Commun. 34, 1-3 (1980). [CrossRef]
  11. J. H. McLeod, "The axicon: a new type of optical element," J. Opt. Soc. Am. 44, 592-597 (1953). [CrossRef]
  12. M. Rioux, R. Tremblay, and P. A. Bélanger, "Linear, annular, and radial focusing with axicons and applications to laser machining," Appl. Opt. 17, 1532-1536 (1978). [CrossRef] [PubMed]
  13. R. M. Herman and T. A. Wiggins, "High-efficiency diffractionless beams of constant size and intensity," Appl. Opt. 33, 7297-7306 (1994). [CrossRef] [PubMed]
  14. J. Dumin, J. J. Miceli, Jr., and J. H. Eberly, "Diffraction-free beams," Phys. Rev. Lett. 58, 1499-1501 (1987). [CrossRef]
  15. A. Vasara, J. Turunen, and A. T. Friberg, "Realization of general nondiffracting beams with computer-generated holograms," J. Opt. Soc. Am. A 6, 1748-1754 (1989). [CrossRef] [PubMed]
  16. Z. Ding, H. Ren, Y. Zhao, J. S. Nelson, and Z. Chen, "High-resolution optical coherence tomography over a large depth range with an axicon lens," Opt. Lett. 27, 243-245 (2002). [CrossRef]
  17. D. McGloin and K. Dholakia, "Bessel beams: diffraction in a new light," Contemp. Phys. 46, 15-28 (2005). [CrossRef]
  18. H. Little, C. T. A. Brown, V. Garcés-Chávez, W. Sibbett, and K. Dholakia, "Optical guiding of microscopic particles in femtosecond and continuous wave Bessel light beams," Opt. Express 12, 2560-2563 (2004). [CrossRef] [PubMed]
  19. V. Garcés-Chavez, D. McGloin, H. Melville, W. Sibbett, and K. Dholakia, "Simultaneous micromanipulation in multiple planes using a self-reconstructing light beam," Nature 419, 145-147 (2002). [CrossRef] [PubMed]
  20. F. W. J. Olver, Asymptotics and Special Functions (Academic, 1974), p. 563.
  21. R. Grunwald, V. Kebbel, U. Griebner, U. Neumann, A. Kummrow, M. Rini, E. T. J. Nibbering, M. Piché, G. Rousseau, and M. Fortin, "Generation and characterization of spatially and temporally localized few-cycle optical wavepackets," Phys. Rev. A 67, 063820 (2003). [CrossRef]
  22. F. Helmchen and W. Denk, "Deep tissue two-photon microscopy," Nat. Methods 2, 932-940 (2005). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article

OSA is a member of CrossRef.

CrossCheck Deposited