OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 5 — May. 17, 2007

Correlation between optical coherence tomography images and histology of pigskin

Roman Kuranov, Veronika Sapozhnikova, Donald Prough, Inga Cicenaite, and Rinat Esenaliev  »View Author Affiliations

Applied Optics, Vol. 46, Issue 10, pp. 1782-1786 (2007)

View Full Text Article

Enhanced HTML    Acrobat PDF (1415 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



Noninvasive imaging techniques such as optical coherence tomography (OCT) are being widely used for early diagnostics of a variety of pathologies. Traditional tissue preparation for histological evaluation alters the dimensions of histological images such that differences between the dimensions of histologic and OCT images can be as great as 2.5-fold. This discrepancy complicates quantitative interpretation of OCT images. Here we used a steel ring sewn to the pigskin to avoid tissue deformation due to excision and fixation and used a surgical suture as a fixed tissue marker. With these techniques, we achieved good correlation between OCT and histological images with differences of less than 10%. The resulting method significantly enhances the diagnostic capabilities of the OCT technique.

© 2007 Optical Society of America

OCIS Codes
(110.2960) Imaging systems : Image analysis
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine

ToC Category:
Optical Coherence Tomography

Original Manuscript: July 3, 2006
Manuscript Accepted: October 27, 2006
Published: March 13, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

Roman Kuranov, Veronika Sapozhnikova, Donald Prough, Inga Cicenaite, and Rinat Esenaliev, "Correlation between optical coherence tomography images and histology of pigskin," Appl. Opt. 46, 1782-1786 (2007)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991). [CrossRef] [PubMed]
  2. M. E. Brezinski and J. G. Fujimoto, "Optical coherence tomography: high-resolution imaging in nontransparent tissue," IEEE J. Sel. Top. Quantum Electron. 5, 1185-1192 (1999). [CrossRef]
  3. J. M. Schmitt, "Optical coherence tomography (OCT): a review," IEEE J. Sel. Top. Quantum Electron. 5, 1205-1215 (1999). [CrossRef]
  4. M. Gloesmann, B. Hermann, C. Schubert, H. Sattmann, P. K. Ahnelt, and W. Drexler, "Histologic correlation of pig retina radial stratification with ultrahigh-resolution optical coherence tomography," Invest. Ophthalmol. Visual Sci. 44, 1696-1702 (2003). [CrossRef]
  5. S. A. Boppart, M. E. Brezinski, C. Pitris, and J. G. Fujimoto, "Optical coherence tomography for neurosurgical imaging of human intracortical melanoma," Neurosurgery 43, 834-841 (1998). [CrossRef] [PubMed]
  6. F. G. Bechara, T. Gambichler, M. Stucker, A. Orlikov, S. Rotterdam, P. Altmeyer, and K. Hoffmann, "Histomorphologic correlation with routine histology and optical coherence tomography," Skin Res. Technol. 10, 169-173 (2004). [CrossRef] [PubMed]
  7. N. D. Gladkova, G. A. Petrova, N. K. Nikulin, S. G. Radenska-Lopovok, L. B. Snopova, Y. P. Chumakov, V. A. Nasonova, V. M. Gelikonov, G. V. Gelikonov, R. V. Kuranov, A. M. Sergeev, and F. I. Feldchtein, "In vivo optical coherence tomography imaging of human skin: norm and pathology," Skin Res. Technol. 6, 6-16 (2000). [CrossRef]
  8. A. I. Kholodnykh, I. Y. Petrova, K. V. Larin, M. Motamedi, and R. O. Esenaliev, "Precision of measurement of tissue optical properties with optical coherence tomography," Appl. Opt. 42, 3027-3037 (2003). [CrossRef] [PubMed]
  9. R. O. Esenaliev, K. V. Larin, I. V. Larina, and M. Motamedi, "Noninvasive monitoring of glucose concentration with optical coherence tomography," Opt. Lett. 26, 992-994 (2001). [CrossRef]
  10. D. Hopwood, "Fixation and fixatives," in Theory and Practice of Histological Techniques, J.D.Bancroft and M.Gamble, eds. (Churchill Livingstone, 2002), pp. 71-72.
  11. V. V. Sapozhnikova, N. M. Shakhova, V. A. Kamensky, S. A. Petrova, L. B. Snopova, and R. V. Kuranov, "Capabilities of fluorescence spectroscopy using 5-ALA and optical coherence tomography for diagnosis of neoplastic processes in the uterine cervix and vulva," Laser Phys. 15, 1664-1673 (2005).
  12. P. Nghiem, P. H. Mckee, and H. A. Haynes, "Merkel cell (cutaneous neuroendocrine) carcinoma," in Atlas of Clinical Oncology: Skin Cancer, A.J.Sober and F.H.Haluska, eds. (BC Decker Inc., 2001), pp. 132-133.
  13. N. M. Shakhova, V. M. Gelikonov, V. A. Kamensky, R. V. Kuranov, and I. V. Turchin, "Clinical aspects of the endoscopic optical coherence tomography and the ways for improving its diagnostic value," Laser Phys. 12, 617-626 (2002).
  14. A. M. Sergeev, V. M. Gelikonov, G. V. Gelikonov, F. I. Feldchtein, R. V. Kuranov, N. D. Gladkova, N. M. Shakhova, L. B. Snopova, A. V. Shakhov, I. A. Kuznetzova, A. N. Denisenko, V. V. Pochinko, Yu. P. Chumakov, and O. S. Streltzova, "In vivo endoscopic OCT imaging of precancer and cancer states of human mucosa," Opt. Express 1, 432-440 (1997). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited