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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 5 — May. 17, 2007

Long-term optical imaging of intrinsic signals in anesthetized and awake monkeys

Anna W. Roe  »View Author Affiliations

Applied Optics, Vol. 46, Issue 10, pp. 1872-1880 (2007)

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Some exciting new efforts to use intrinsic signal optical imaging methods for long-term studies in anesthetized and awake monkeys are reviewed. The development of such methodologies opens the door for studying behavioral states such as attention, motivation, memory, emotion, and other higher-order cognitive functions. Long-term imaging is also ideal for studying changes in the brain that accompany development, plasticity, and learning. Although intrinsic imaging lacks the temporal resolution offered by dyes, it is a high spatial resolution imaging method that does not require application of any external agents to the brain. The bulk of procedures described here have been developed in the monkey but can be applied to the study of surface structures in any in vivo preparation.

© 2007 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(330.7310) Vision, color, and visual optics : Vision

ToC Category:
Optics in neuroscience

Original Manuscript: July 7, 2006
Revised Manuscript: October 31, 2006
Manuscript Accepted: November 3, 2006
Published: March 13, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

Anna W. Roe, "Long-term optical imaging of intrinsic signals in anesthetized and awake monkeys," Appl. Opt. 46, 1872-1880 (2007)

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  1. T. Bonhoeffer and A. Grinvald, "Optical imaging based on intrinsic signals: the methodology," in Brain Mapping. The Methods, A. W. Toga and J. C. Mazziota, eds. (Academic, 1996), pp. 55-87.
  2. I. Vanzetta and A. Grinvald, "Increased cortical oxidative metabolism due to sensory stimulation: implications for functional brain imaging," Science 286, 1555-1558 (1999). [CrossRef] [PubMed]
  3. D. S. Kim, T. Q. Duong, and S. G. Kim, "High-resolution mapping of iso-orientation columns by fMRI," Nat. Neurosci. 3, 164-169 (2000). [CrossRef] [PubMed]
  4. A. F. Cannestra, N. Pouratian, S. Y. Bookheimer, N. A. Martin, D. P. Beckerand, and A. W. Toga, "Temporal spatial differences observed by functional MRI and human intraoperative optical imaging," Cereb. Cortex 11, 773-782 (2001). [CrossRef] [PubMed]
  5. A. Devor, A. K. Dunn, M. L. Andermann, I. Ulbert, D. A. Boas, and A. M. Dale, "Coupling of total hemoglobin concentration, oxygenation, and neural activity in rat somatosensory cortex," Neuron 39, 353-359 (2003). [CrossRef] [PubMed]
  6. J. K. Thompson, M. R. Peterson, and R. D. Freeman, "Single-neuron activity and tissue oxygenation in the cerebral cortex," Science 299, 1070-1072 (2003). [CrossRef] [PubMed]
  7. S. Sheth, M. Nemoto, M. Guiou, M. Walker, N. Pouratian, and A. W. Toga, "Evaluation of coupling between optical intrinsic signals and neuronal activity in rat somatosensory cortex," Neuroimage 19, 884-894 (2003). [CrossRef] [PubMed]
  8. A. Zepeda, C. Ariasa, and F. Sengpiel, "Optical imaging of intrinsic signals: recent developments in the methodology and its applications," J. Neurosci. Methods 136, 1-21 (2004). [CrossRef] [PubMed]
  9. A. W. Roe, "Modular complexity of Area V2 in the Macaque monkey," in The Primate Visual System, C. Collins and J. H. Kaas, eds. (CRC, 2003), pp. 109-138.
  10. A. Grinvald, R. D. Frostig, R. M. Siegel, and E. Bartfeld, "High-resolution optical imaging of functional brain architecture in the awake monkey," Proc. Natl. Acad. Sci. U.S.A. 88, 11559-11563 (1991). [CrossRef] [PubMed]
  11. N. Vnek, R. M. Ramsden, C. P. Hung, P. S. Goldman-Rakic, and A. W. Roe, "Optical imaging of functional domains in the cortex of the awake and behaving monkey," Proc. Natl. Acad. Sci. U.S.A. 96, 4057-4060 (1999). [CrossRef] [PubMed]
  12. E. Seidemann, A. Arieli, A. Grinvald, and H. Slovin, "Dynamics of depolarization and hyperpolarization in the frontal cortex and saccade goal," Science 295, 862-865 (2002). [CrossRef] [PubMed]
  13. A. W. Roe, D. Walled, E. Sybirska, and P. S. Goldman-Rakic, "Optical imaging of prefrontal cortex during oculomotor delay response task in Macaque monkey," Soc. Neurosci. Abstract , San Diego, Calif. (2004).
  14. R. M. Siegel, M. Raffi, R. E. Phinney, J. A. Turner, and G. Jando, "Functional architecture of eye position gain fields in visual association cortex of behaving monkey," J. Neurophysiol. 90, 1279-1294 (2003). [CrossRef] [PubMed]
  15. R. D. Frostig, E. E. Lieke, D. Y. Ts'o, and A. Grinvald, "Cortical functional architecture and local coupling between neuronal activity and the microcirculation revealed by in vivo high-resolution optical imaging of intrinsic signals," Proc. Natl. Acad. Sci. U.S.A. 87, 6082-6086 (1990). [CrossRef] [PubMed]
  16. A. F. Cannestra, N. Pouratian, M. H. Shomer, and A. W. Toga, "Refractory periods observed by intrinsic signal and fluorescent dye imaging," J. Neurophysiol. 80, 1522-1532 (1998). [PubMed]
  17. W. H. Bosking, R. Kretz, M. L. Pucak, and D. Fitzpatrick, "Functional specificity of callosal connections in tree shrew striate cortex," J. Neurosci. 20, 2346-2359 (2000). [PubMed]
  18. D. M. Rector, R. F. Rogers, and J. S. George, "A focusing image probe for assessing neural activity in vivo," J. Neurosci. Methods 91, 135-145 (1999). [CrossRef] [PubMed]
  19. E. Shtoyerman, A. Arieli, H. Slovin, I. Vanzetta, and A. Grinvald, "Long-term optical imaging and spectroscopy reveal mechanisms underlying the intrinsic signal and stability of cortical maps in VI of behaving monkeys," J. Neurosci. 20, 8111-8121 (2000). [PubMed]
  20. L. M. Chen, B. Heider, F. L. Healy, B. M. Ramsden, G. V. Williams, and A. W. Roe, "A chamber and artificial dura method for long-term optical imaging in primates," J. Neurosci. Methods 113, 41-49 (2002). [CrossRef]
  21. D. E. Sakas, K. Chamvises, L. F. Borges, and N. T. Zervas, "Biologically inert synthetic dural substitutes. Appraisal of a medical-grade aliphatic polyurethane and a polysiloxane-carbonate block copolymer," J. Neurosurg. 73, 936-941 (1990). [CrossRef] [PubMed]
  22. A. W. Roe, F. L. Healy, R. M. Friedman, B. Heider, and L. M. Chen, "Differences in SI topography between anesthetized and awake squirrel monkey as revealed by optical imaging," Program No. 28:651.3. 2002 Abstract Viewer/Itinerary Planner. Washington, D.C.: Society for Neuroscience, CD-ROM (2002).
  23. B. Heider, L. M. Chen, G. V. Williams, F. L. Healy, B. M. Ramsden, and A. W. Roe, "Chronic optical imaging in anesthetized and alert monkeys," Soc. Neurosci. Abstract , 619.33 (2001).
  24. G. M. Ghose and D. Y. Ts'o, "Form processing modules in primate area V4," J. Neurophysiol. 77, 2191-2196 (1997). [PubMed]
  25. X. Xu, W. Bosking, G. Sary, J. Stefansic, D. Shima, and V. Casagrande, "Functional organization of visual cortex in the owl monkey," J. Neurosci. 24, 6237-6247 (2004). [CrossRef] [PubMed]
  26. S. T. Schuett, T. Bonhoeffer, and M. Hubener, "Pairing-induced changes of orientation maps in cat visual cortex," Neuron 32, 325-337 (2001). [CrossRef] [PubMed]
  27. B. M. Ramsden, C. P. Hung, and A. W. Roe, "Real and illusory contour processing in Area V1 of the primate--a cortical balancing act," Cerebral Cortex 11, 648-665 (2001). [CrossRef] [PubMed]
  28. L. M. Chen, R. M. Friedman, B. M. Ramsden, R. H. LaMotte, and A. W. Roe, "Fine-scale organization of primary somatosensory cortex (Area 3b) in the squirrel monkey revealed with intrinsic optical imaging," J. Neurophysiol. 86, 3011-3029 (2001). [PubMed]
  29. H. Slovin, A. Arieli, R. Hildesheim, and A. Grinvald, "Long-term voltage-sensitive dye imaging reveals cortical dynamics in behaving monkeys," J. Neurophysiol. 88, 3421-3438 (2002). [CrossRef] [PubMed]
  30. G. Wang, M. Tanifuji, and K. Tanaka, "Functional architecture in monkey inferotemporal cortex revealed by in vivo optical imaging," Neurosci. Res. (NY) 32, 33-46 (1998). [CrossRef] [PubMed]
  31. M. Tommerdahl, K. A. Delemos, O. V. Favorov, C. B. Metz, C. J. Vierck, and B. L. Whitsel, "Response of anterior parietal cortex to different modes of same-site skin stimulation," J. Neurophysiol. 80, 3272-3283 (1998). [PubMed]
  32. L. Sirovich and E. Kaplan, "Analysis methods for optical imaging," in Optical Imaging: CRC Reviews, R.Frostig, ed. (CRC, 2002).
  33. T. H. Schwartz, L. M. Chen, R. M. Friedman, D. D. Spencer, and A. W. Roe, "Intraoperative optical imaging of face topography in human somatosensory cortex," NeuroReport 15, 1527-1532 (2004). [CrossRef] [PubMed]
  34. D. C. Lyon, X. Xu, V. A. Casagrande, J. D. Stefansic, D. Shima, and J. H. Kaas, "Optical imaging reveals retinotopic organization of dorsal V3 in New World owl monkeys," Proc. Natl. Acad. Sci. U.S.A. 99, 15735-15742 (2002). [CrossRef] [PubMed]
  35. R. W. Dykes, M. Sur, M. M. Merzenich, J. H. Kaas, and R. J. Nelson, "Regional segregation of neurons responding to quickly adapting, slowly adapting, deep and pacinian receptors within thalamic ventroposterior lateral and ventroposterior inferior nuclei in the squirrel monkey (Saimiri sciureus)," Neurosci. 6, 1687-1692 (1981). [CrossRef]
  36. Y. Iwamura, M. Tanaka, M. Sakamoto, and O. Hikosaka, "Converging patterns of finger representation and complex response properties of neurons in Area 1 of the first somatoensory cortex of the conscious monkey," Exp. Brain Res. 51, 327-337 (1983). [CrossRef]
  37. K. D. Alloway and H. Burton, "Differential effects of GABA and bicuculline on rapidly- and slowly-adapting neurons in primary somatosensory cortex of primates," Exp. Brain Res. 85, 98-610 (1991). [CrossRef]
  38. C. E. Schroeder, S. Seto, J. C. Arezzo, and P. E. Garraghty, "Electrophysiological evidence for overlapping dominant and latent inputs to somatosensory cortex in squirrel monkeys," J. Neurophysiol. 74, 722-732 (1995). [PubMed]
  39. S. Funahashi, C. J. Bruce, and P. S. Goldman-Rakic, "Mnemonic coding of visual space in the monkey's dorsolateral prefrontal cortex," J. Neurophysiol. 61, 331-349 (1989). [PubMed]
  40. R. M. Siegel and H. L. Read, "Analysis of optic flow in the monkey parietal area 7a," Cereb. Cortex 7, 327-346 (1997). [CrossRef] [PubMed]
  41. M. Raffi and R. M. Siegel, "Functional architecture of spatial attention in the parietal cortex of the behaving monkey," J. Neurosci. 25, 5171-586 (2005). [CrossRef] [PubMed]

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