OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 5 — May. 17, 2007

Analysis of laser-induced fluorescence spectra of in vitro plant tissue cultures

Ana Celia Muñoz-Muñoz, Humberto Gutiérrez-Pulido, José Manuel Rodríguez-Domínguez, Antonia Gutiérrez-Mora, Benjamín Rodríguez-Garay, and Jesús Cervantes-Martínez  »View Author Affiliations


Applied Optics, Vol. 46, Issue 11, pp. 2138-2142 (2007)
http://dx.doi.org/10.1364/AO.46.002138


View Full Text Article

Enhanced HTML    Acrobat PDF (861 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We demonstrate the effectiveness of laser-induced fluorescence (LIF) for monitoring the development and stress detection of in vitro tissue cultures in a nondestructive and noninvasive way. The changes in LIF spectra caused by the induction of organogenesis, the increase of the F690∕F740 ratio as a result of the stress originated in the organogenic explants due to shoot emergence, and the relationship between fluorescence spectra and shoot development were detected by LIF through closed containers of Saintpaulia ionantha.

© 2007 Optical Society of America

OCIS Codes
(300.2140) Spectroscopy : Emission
(300.2530) Spectroscopy : Fluorescence, laser-induced
(300.6170) Spectroscopy : Spectra
(300.6550) Spectroscopy : Spectroscopy, visible

ToC Category:
Spectroscopy

History
Original Manuscript: January 27, 2006
Revised Manuscript: September 12, 2006
Manuscript Accepted: November 6, 2006
Published: March 20, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Ana Celia Muñoz-Muñoz, Humberto Gutiérrez-Pulido, José Manuel Rodríguez-Domínguez, Antonia Gutiérrez-Mora, Benjamín Rodríguez-Garay, and Jesús Cervantes-Martínez, "Analysis of laser-induced fluorescence spectra of in vitro plant tissue cultures," Appl. Opt. 46, 2138-2142 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-46-11-2138


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. W. Lüdeker, H. G. Dahn, and K. P. Günther, "Detection of fungal infection of plants by laser-induced fluorescence: an attempt to use remote sensing," J. Plant Physiol. 148, 579-585 (1996).
  2. L. Taiz and E. Zeiger, "Photosynthesis: the light reactions," in Plant Physiology, A.D. Sinauer, ed. (Sinauer, 1998), pp. 155-193.
  3. H. K. Lichtenthaler, "In vivo chlorophyll fluorescence as a tool for stress detection in plants," in Applications of Chlorophyll Fluorescence in Photosynthesis Research, Stress Physiology, Hydrobiology and Remote Sensing, H. K. Lichtenthaler, ed. (Kluwer, 1988), pp. 129-142.
  4. K. P. Günther, H. G. Dahn, and W. Lüdeker, "Remote sensing vegetation status by laser-induced fluorescence," Remote Sens. Environ. 47, 10-17 (1994). [CrossRef]
  5. J. C. McFarlane, R. D. Watson, A. F. Theisen, R. D. Jackson, W. L. Ehrler, P. J. Pinter, Jr., S. B. Idso, and R. J. Reginato, "Plant stress detection by remote measurement of fluorescence," Appl. Opt. 19, 3287-3289 (1980). [CrossRef] [PubMed]
  6. H. K. Lichtenthaler, "The Kaustky effect: 60 years of chlorophyll fluorescence induction kinetics," Photosynthetica 27, 45-55 (1992).
  7. H. K. Lichtenthaler and U. Rinderle, "The role of chlorophyll fluorescence in detection of stress in plants," Crit. Rev. Anal. Chem. Suppl. I 19, 529-585 (1988).
  8. N. Subhash and C. N. Mohanan, "Laser-induced red chlorophyll fluorescence signatures as nutrient stress indicator in rice plants," Remote Sens. Environ. 47, 45-50 (1994). [CrossRef]
  9. J. Cervantes-Martínez, R. Flores-Hernández, B. Rodríguez-Garay, and F. Santacruz-Ruvalcaba, "Detection of bacterial infection of agave plants by laser-induced fluorescence," Appl. Opt. 41, 2541-2545 (2002). [CrossRef] [PubMed]
  10. S. Balachandran, V. M. Hurry, S. E. Kelley, C. B. Osmond, S. A. Robinson, J. Rohozinski, G. G. R. Seaton, and D. A. Sims, "Concepts of plant biotic stress. Some insights into the stress physiology of virus-infected plants, from the perspective of photosynthesis," Physiol. Plant. 100, 203-213 (1997). [CrossRef]
  11. J. Cervantes-Martínez, S. López-Díaz, and B. Rodríguez-Garay, "Detection of the effects of Methylobacterium in Agave tequilana Weber var. azul by laser-induced fluorescence," Plant Sci. 166, 889-892 (2004). [CrossRef]
  12. V. Goltsev, T. Genkov, M. Lexa, and I. Ivanova, "Effect of benzyladenine, 4-PU-30 and thidiazuron on millisecond delayed and prompt chlorophyll fluorescence of Dianthus caryophyllus L. axillary buds cultured in vitro," Sci. Hortic. 89, 41-54 (2001). [CrossRef]
  13. P. Kadlecek, B. Rank, and I. Tichá, "Photosynthesis and photoprotection in Nicotiana tabacum L. in vitro-grown plantlets," J. Plant Physiol. 160, 1017-1024 (2003). [CrossRef] [PubMed]
  14. R. J. Mathias and L. A. Boyd, "Cefotaxime stimulates callus growth, embryogenesis and regeneration in hexaploid bread wheat," Plant Sci. 46, 217-223 (1986). [CrossRef]
  15. L. M. Yeppes and H. S. Aldwinckle, "Factors that affect leaf regeneration efficiency in apple, and effect of antibiotics in morphogenesis," Plant Cell Tiss. Org. Cult. 37, 257-269 (1994).
  16. B. S. Bhau and A. K. Wakhlu, "Effect of some antibiotics on the in vitro morphogenetic response from callus cultures of Coryphantha elephantidens," Biol. Plant. 44, 19-24 (2001). [CrossRef]
  17. S. A. Danilova and Y. I. Dolgikh, "The stimulatory effect of the antibiotic cefotaxime on plant regeneration in maize tissue culture," Russ. J. Plant Physiol. 51, 559-562 (2004). [CrossRef]
  18. P. Holford and H. J. Newbury, "The effects of antibiotics and their breakdown products on the in vitro growth of Antirrhinum majus," Plant Cell Rep. 11, 93-96 (1992). [CrossRef]
  19. N. Barbarin, B. Tilquin, and E. de Hoffmann. "Radiosterilization of cefotaxime: investigation of potential degradation compounds by liquid chromatography-electrospray mass spectrometry," J. Chromatogr. A 929, 51-61 (2001). [CrossRef]
  20. Q. Zhang, J. T. Wiskich, and K. I. Woole, "Respiratory activities in chloromphenicol-treated tobacco cells," Physiol. Plant. 105, 224-232 (1999). [CrossRef]
  21. B. M. Reed, P. M. Buckley, and T. N. De Wilde, "Detection and eradication of endophytic bacteria from micropropagated mint plants," In Vitro Cell Dev. Biol. 31, 53-57 (1995).
  22. T. A. Murashige and F. C. Skoog, "A revised medium for rapid growth and bioassay with tobacco tissue cultures," Physiol. Plant. 15, 473-497 (1962). [CrossRef]
  23. G. C. Phillips and G. B. Collins, "In vitro tissue culture of selected legumes and plant regeneration from callus cultures of red clover," Crop Sci. 19, 59-64 (1979). [CrossRef]
  24. U. Rinderle and H. K. Lichtenthaler, "The chlorophyll fluorescence ratio F690/F735 as a possible stress indicator," in Applications of Chlorophyll Fluorescence in Photosynthesis Research, Stress Physiology, Hydrobiology and Remote Sensing, H. K. Lichtenthaler, ed. (Kluwer, 1988), pp. 189-196.
  25. E. W. Chapelle, J. E. McMurtrey III, F. M. Wood, and W. W. Newcomb, "Laser-induced fluorescence of green plants. 2: LIF caused by nutrient deficiencies in corn," Appl. Opt. 23, 139-142 (1984). [CrossRef]
  26. E. F. George, Plant Propagation by Tissue Culture. The Technology. Part I. (Exegetics Limited, 1993).
  27. D. J. Donelly, W. E. Vidaver, and K. Colbow, "Fixation of 14CO2 in tissue cultured raspberry prior to and after transfer to soil," Plant Cell Tiss. Org. Cult. 3, 313-317 (1984). [CrossRef]
  28. B. W. W. Grout and M. J. Aston, "Transplanting of cauliflower plants regenerated from meristem culture. II: Carbon dioxide fixation and the development of photosynthetic ability," Hortic. Res. 17, 65-71 (1978).
  29. J. Mithila, J. C. Hall, J. M. R. Victor, and P. K. Saxena, "Thidiazuron induces shoot organogenesis at low concentrations and somatic embryogenesis at high concentrations on leaf and petiole explants of African violet (Saintpaulia ionantha Wendl)," Plant Cell Rep. 21, 408-414 (2003). [PubMed]
  30. C. Buschmann and H. K. Lichtenthaler, "Principles and characteristics of multi-color fluorescence imaging of plants," J. Plant Physiol. 152, 297-314 (1998).
  31. B. S. Mangat, M. K. Pelekis, and A. C. Cassells, "Changes in the starch content during organogenesis in in vitro cultured Begonia rex stem explants," Physiol. Plant. 79, 267-274 (1990). [CrossRef]
  32. F. Stober, M. Lang, and H. K. Lichtenthaler, "Blue, green, and red fluorescence emission signatures of green, etiolated, and white leaves," Remote Sens. Environ. 47, 65-71 (1994). [CrossRef]
  33. N. R. Baker and R. J. Strasser, "Development and spectral characteristics of excitation energy transfer between the photosystems during chloroplast biogenesis," Photobiochem. Photobiophys. 4, 265-273 (1982).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 

« Previous Article

OSA is a member of CrossRef.

CrossCheck Deposited