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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 5 — May. 17, 2007

Two-dimensional biosensor arrays based on surface plasmon resonance phase imaging

C. L. Wong, H. P. Ho, T. T. Yu, Y. K. Suen, Winnie W. Y. Chow, S. Y. Wu, W. C. Law, W. Yuan, W. J. Li, S. K. Kong, and Chinlon Lin  »View Author Affiliations

Applied Optics, Vol. 46, Issue 12, pp. 2325-2332 (2007)

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We present a biosensor design based on capturing the two-dimenstional (2D) phase image of surface plasmon resonance (SPR). This 2D SPR imaging technique may enable parallel label-free detection of multiple analytes and is compatible with the microarray chip platform. This system uses our previously reported differential phase measurement approach, in which 2D phase maps obtained from the signal (P) and reference (S) polarizations are compared pixel by pixel. This technique greatly improves detection resolution as the subtraction step can eliminate measurement fluctuations caused by external disturbances as they essentially appear in both channels. Unlike conventional angular SPR systems, in which illumination from a range of angles must be used, phase measurement requires illumination from only one angle, thus making it well suited for 2D measurement. Also, phase-stepping introduced from a moving mirror provides the necessary modulation for accurate detection of the phase. In light of the rapidly increasing need for fast real-time detection, quantification, and identification of a range of proteins for various biomedical applications, our 2D SPR phase imaging technique should hold a promising future in the medical device market.

© 2007 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(240.6680) Optics at surfaces : Surface plasmons

ToC Category:
Imaging Systems

Original Manuscript: September 6, 2006
Revised Manuscript: November 30, 2006
Manuscript Accepted: December 1, 2006
Published: April 3, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

C. L. Wong, H. P. Ho, T. T. Yu, Y. K. Suen, Winnie W. Y. Chow, S. Y. Wu, W. C. Law, W. Yuan, W. J. Li, S. K. Kong, and Chinlon Lin, "Two-dimensional biosensor arrays based on surface plasmon resonance phase imaging," Appl. Opt. 46, 2325-2332 (2007)

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