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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 5 — May. 17, 2007

Combined enhanced fluorescence and label-free biomolecular detection with a photonic crystal surface

Patrick C. Mathias, Nikhil Ganesh, Leo L. Chan, and Brian T. Cunningham  »View Author Affiliations

Applied Optics, Vol. 46, Issue 12, pp. 2351-2360 (2007)

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A 2D photonic crystal surface with a different period in each lateral direction is demonstrated to detect biomolecules using two distinct sensing modalities. The sensing mechanisms both rely on the generation of a resonant reflection peak at one of two specific wavelengths, depending on the polarization of light that is incident on the photonic crystal. One polarization results in a resonant reflection peak in the visible spectrum to coincide with the excitation wavelength of a fluorophore, while the orthogonal polarization results in a resonant reflection peak at an infrared wavelength which is used for label-free detection of adsorbed biomolecules. The photonic crystal resonance for fluorescence excitation causes enhanced near fields at the structure surface, resulting in increased signal from fluorophores within 100 nm of the device surface. Label-free detection is performed by illuminating the photonic crystal with white light and monitoring shifts in the peak reflected wavelength of the infrared resonance with a high-resolution imaging detection instrument. Rigorous coupled-wave analysis was used to determine optimal dimensions for the photonic crystal structure, and devices were fabricated using a polymer-based nanoreplica molding approach. Fluorescence-based and label-free detection were demonstrated using arrays of spots of dye-conjugated streptavidin. Quantification of the fluorescent signal showed that the fluorescence output from protein spots on the photonic crystal was increased by up to a factor of 35, and deposited spots were also imaged in the label-free detection mode.

© 2007 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(230.1950) Optical devices : Diffraction gratings

ToC Category:
Optical Devices

Original Manuscript: August 16, 2006
Revised Manuscript: November 20, 2006
Manuscript Accepted: December 14, 2006
Published: April 3, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

Patrick C. Mathias, Nikhil Ganesh, Leo L. Chan, and Brian T. Cunningham, "Combined enhanced fluorescence and label-free biomolecular detection with a photonic crystal surface," Appl. Opt. 46, 2351-2360 (2007)

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