Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Adaptively controlled supercontinuum pulse from a microstructure fiber for two-photon excited fluorescence microscopy

Not Accessible

Your library or personal account may give you access

Abstract

Selective fluorescence excitation of specific molecular species is demonstrated by using coherent control of two-photon excitation with supercontinuum pulses generated with a microstructure fiber. Pulse shaping prior to pulse propagation through the fiber is controlled by a self-learning optimization loop so that the highest fluorescence signal contrast between two fluorescent proteins is obtainable. The self-learning optimization loop successfully controls both the optical nonlinarity of the microstructure fiber and the two-photon excitation of the fluorescent proteins.

© 2007 Optical Society of America

Full Article  |  PDF Article
More Like This
Photon-number squeezing in a solitonlike Raman Stokes component during propagation of ultrashort pulses in a microstructure fiber

Atsushi Tada, Kenichi Hirosawa, Fumihiko Kannari, Masahiro Takeoka, and Masahide Sasaki
J. Opt. Soc. Am. B 24(3) 691-698 (2007)

Compact multicolor two-photon fluorescence microscopy enabled by tailorable continuum generation from self-phase modulation and dispersive wave generation

Lu-Ting Chou, Shao-Hsuan Wu, Hao-Hsuan Hung, Wei-Zong Lin, Zi-Ping Chen, Anatoly A. Ivanov, and Shih-Hsuan Chia
Opt. Express 30(22) 40315-40327 (2022)

Temporal control of local plasmon distribution on Au nanocrosses by ultra-broadband femtosecond laser pulses and its application for selective two-photon excitation of multiple fluorophores

Takuya Harada, Keiichiro Matsuishi, Yu Oishi, Keisuke Isobe, Akira Suda, Hiroyuki Kawan, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa, and Fumihiko Kannari
Opt. Express 19(14) 13618-13627 (2011)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (9)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (1)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved