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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 9 — Sep. 26, 2007

Scattering properties of microalgae: the effect of cell size and cell wall

Øyvind Svensen, Øyvind Frette, and Svein Rune Erga  »View Author Affiliations

Applied Optics, Vol. 46, Issue 23, pp. 5762-5769 (2007)

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The main objective of this work was to investigate how the cell size and the presence of a cell wall influence the scattering properties of the green microalgae Chlamydomonas reinhardtii. The growth cycle of two strains, one with a cell wall and one without, was synchronized to be in the same growth phase. Measurements were conducted at two different phases of the growth cycle on both strains of the algae. It was found that the shape of the scattering phase function was very similar for both strains at both growth phases, but the regular strain with a cell wall scatters more strongly than the wall-less mutant. It was also found that the mutant strain has a stronger increase in scattering than the regular strain, as the algae grow, and that the scattering from the regular strain is more wavelength dependent than from the mutant strain.

© 2007 Optical Society of America

OCIS Codes
(010.4450) Atmospheric and oceanic optics : Oceanic optics
(290.5820) Scattering : Scattering measurements

ToC Category:

Original Manuscript: December 20, 2006
Revised Manuscript: March 29, 2007
Manuscript Accepted: May 1, 2007
Published: August 8, 2007

Virtual Issues
Vol. 2, Iss. 9 Virtual Journal for Biomedical Optics

Øyvind Svensen, Øyvind Frette, and Svein Rune Erga, "Scattering properties of microalgae: the effect of cell size and cell wall," Appl. Opt. 46, 5762-5769 (2007)

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  1. C. B. Field, M. T. Behrenfeld, J. T. Randerson, and P. Falkowski, "Primary production of the biosphere: Integrating terrestrial and oceanic components," Science 281, 237-240 (1998). [CrossRef] [PubMed]
  2. R. B. Myneni, C. D. Keeling, C. J. Tucker, G. Asrar, and R. R. Nemani, "Increased plant growth in the northern high latitudes from 1981 to 1991," Nature 386, 698-702 (1997). [CrossRef]
  3. W. Eikrem and J. Throndsen, "Toxic prymnesiophytes identified from Norwegian coastal waters," in Toxic Phytoplankton Blooms in the Sea, T. J. Smayda and Y. Shimizu, eds., Proceedings of the Fifth International Conference on the Toxic Marine Phytoplankton (Elsevier, 1993), pp. 687-692.
  4. D. Stramski and C. D. Mobley, "Effects of microbial particles on oceanic optics: A database of single-particles optical properties," Limnol. Oceanogr. 42, 538-549 (1997). [CrossRef]
  5. A. Morel and Y. Ahn, "Optics of heterotrophic nanoflagellates and ciliates: A tentative assessment of their scattering role in oceanic waters compared to those of bacterial and algal cells," N. Z. J. Marine Freshwater Res. 49, 177-202 (1991).
  6. K. J. Voss, W. M. Balch, and K. A. Kilpatrick, "Scattering and attenuation properties of Emiliania huxleyi cells and their detached coccoliths," Limnol. Oceanogr. 43, 870-876 (1998). [CrossRef]
  7. H. R. Gordon and T. Du, "Light scattering by nonspherical particles: Application to coccoliths detached from Emiliania huxleyi," Limnol. Oceanogr. 46, 1438-1454 (2001). [CrossRef]
  8. R. D. Vaillancourt, C. W. Brown, R. R. L. Guillard, and W. M. Balch, "Light backscattering properties of marine phytoplankton: relationships to cell size, chemical composition, and taxonomy," J. Plankton Res. 26, 191-212 (2004). [CrossRef]
  9. T. J. Petzold, "Volume scattering functions for selected ocean waters," in Benchmark Papers in Optics: Light in the Sea, J. Tyler, ed. (Dowden, Hutchinson, and Ross, Inc., 1972), Vol. 3, pp. 152-174.
  10. C. D. Mobley, L. K. Sundman, and E. Boss, "Phase function effects on oceanic light fields," Appl. Opt. 41, 1035-1050 (2002). [CrossRef] [PubMed]
  11. M. E. Lee and M. R. Lewis, "A new method of the measurement of the optical volume scattering function in the upper ocean," J. Atmos. Ocean. Technol. 20, 563-571 (2003). [CrossRef]
  12. H. Volten, F. de Hann, J. W. Hovenier, R. Schreurs, W. Vassen, A. G. Dekker, H. J. Hoogenboom, F. Charlton, and R. Wouts, "Laboratory measurements of angular distributions of light scattered by phytoplankton and slit," Limnol. Oceanogr. 44, 1180-1197 (1998). [CrossRef]
  13. E. H. Harris, The Chlamydomnas Sourcebook--A Comprehensive Guide to Biology and Laboratory Use (Academic, 1989).
  14. T. Lien and G. Knutsen, "Synchronized cultures of a cell wall-less mutant of Chlamydomonas reinhardtii," Arch. Microbiol. 108, 189-194 (1976). [CrossRef] [PubMed]
  15. T. Lien and G. Knutsen, "Synchronous growth of Chlamydomonas reinhardtii (Chlorophyceae): A review of optimal conditions," J. Phycol. 15, 191-200 (1979). [CrossRef]
  16. J. K. Lotsberg, E. Marken, J. J. Stamnes, S. R. Erga, K. Aursland, and C. D. Olseng, "Laboratory measurements of light scattering from marine particles," Limnol. Oceanogr. Methods 5, 34-40 (2007). [CrossRef]
  17. H. van de Hulst, Light Scattering by Small Particles (Wiley, 1957), pp. 143-146.
  18. T. Kaiser and G. Schweiger, "Stable algorithm for the computation of Mie coefficients for scattered and transmitted fields of a coated sphere," Comput. Phys. 7, 682-686 (1993). [CrossRef]
  19. J. C. Kitchen and J. R. V. Zaneveld, "A three-layered sphere model of the optical properties of phytoplankton," Limnol. Oceanogr. 37, 1680-1690 (1992). [CrossRef]
  20. E. Van Donk, M. Lürling, D. O. Hessen, and G. M. Lokhorst, "Altered cell wall morphology in nutrient-deficient phytoplankton and its impact on grazers," Limnol. Oceanogr. 42, 357-364 (1997). [CrossRef]
  21. A. Morel, "Optics of marine particles and marine optics," Particle Analysis in Oceanography, NATO ASI series G27 (1991), pp. 142-188.
  22. Y. Ahn, A. Bricaud, and A. Morel, "Light backscattering efficiency and related properties of some phytoplankters," Deep-Sea Res. 39, 1835-1855 (1992). [CrossRef]
  23. D. Stramski, G. Rosenberg, and L. Legendre, "Photosynthesis and optical properties of the marine chlorophyte Dunaliella tertiolecta grown under fluctuating light caused by surface-wave focusing," Mar. Biol. 115, 363-372 (1993). [CrossRef]
  24. G. Knutsen and T. Lien, "Properties of synchronous cultures of Chlamydomonas reinhardtii under optimal conditions, and some factors influencing them," Ber. Dtsch. Bot. Ges. 94, 599-611 (1981).

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