Modeling biological fluorescence emission spectra using Lorentz line shapes and nonlinear optimization
Applied Optics, Vol. 46, Issue 24, pp. 6192-6195 (2007)
http://dx.doi.org/10.1364/AO.46.006192
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Abstract
Using the Levenberg–Marquardt nonlinear optimization algorithm and a series of Lorentzian line shapes, the fluorescence emission spectra from BG (Bacillus globigii) bacteria can be accurately modeled. This method allows data from both laboratory and field sources to model the return signal from biological aerosols using a typical LIF (lidar induced fluorescence) system. The variables found through this procedure match individual fluorescence components within the biological material and therefore have a physically meaningful interpretation. The use of this method also removes the need to calculate phase angles needed in autoregressive all-pole models.
© 2007 Optical Society of America
OCIS Codes
(070.6020) Fourier optics and signal processing : Continuous optical signal processing
(190.1900) Nonlinear optics : Diagnostic applications of nonlinear optics
(260.2510) Physical optics : Fluorescence
(280.3640) Remote sensing and sensors : Lidar
(300.2530) Spectroscopy : Fluorescence, laser-induced
ToC Category:
Spectroscopy
History
Original Manuscript: September 29, 2006
Revised Manuscript: June 6, 2007
Manuscript Accepted: June 15, 2007
Published: August 20, 2007
Virtual Issues
Vol. 2, Iss. 9 Virtual Journal for Biomedical Optics
Citation
Paul D. Nation, A. Q. Howard, and Lincoln J. Webb, "Modeling biological fluorescence emission spectra using Lorentz line shapes and nonlinear optimization," Appl. Opt. 46, 6192-6195 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-46-24-6192
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