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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 1 — Jan. 29, 2008

Flexible depth-of-field imaging system using a spatial light modulator

Deokhwa Hong, Kangmin Park, Hyungsuck Cho, and Minyoung Kim  »View Author Affiliations

Applied Optics, Vol. 46, Issue 36, pp. 8591-8599 (2007)

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A major problem of optical microscopes is their small depth-of-field (DOF), which hinders automation of micro object manipulation using visual feedback. Wavefront coding, a well-known method for extending DOF, is not suitable for direct application to micro object manipulation systems based on visual feedback owing to its expensive computational cost and due to a trade-off between the DOF and the image resolution properties. To solve such inherent problems, a flexible DOF imaging system using a spatial light modulator in the pupil plane is proposed. Especially, the trade-off relationship is quantitatively analyzed by experiments. Experimental results show that, for low criterion resolution, the DOF increases as the strength of the mask increases, while such a trend was not found for high criterion resolution. With high criterion resolution, the DOF decreases as the mask strength increases when high-resolution images are required. The results obtained can be used effectively to find the optimum mask strength given the desired image resolution.

© 2007 Optical Society of America

OCIS Codes
(100.1830) Image processing : Deconvolution
(100.5090) Image processing : Phase-only filters
(110.1220) Imaging systems : Apertures
(150.0150) Machine vision : Machine vision
(220.2560) Optical design and fabrication : Propagating methods
(230.3720) Optical devices : Liquid-crystal devices

ToC Category:
Image Processing

Original Manuscript: May 14, 2007
Revised Manuscript: September 24, 2007
Manuscript Accepted: October 5, 2007
Published: December 14, 2007

Virtual Issues
Vol. 3, Iss. 1 Virtual Journal for Biomedical Optics

Deokhwa Hong, Kangmin Park, Hyungsuck Cho, and Minyoung Kim, "Flexible depth-of-field imaging system using a spatial light modulator," Appl. Opt. 46, 8591-8599 (2007)

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