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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 3 — Mar. 7, 2007

Scanning holographic microscopy with resolution exceeding the Rayleigh limit of the objective by superposition of off-axis holograms

Guy Indebetouw, Yoshitaka Tada, Joseph Rosen, and Gary Brooker  »View Author Affiliations


Applied Optics, Vol. 46, Issue 6, pp. 993-1000 (2007)
http://dx.doi.org/10.1364/AO.46.000993


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Abstract

We present what we believe to be a new application of scanning holographic microscopy to superresolution. Spatial resolution exceeding the Rayleigh limit of the objective is obtained by digital coherent addition of the reconstructions of several off-axis Fresnel holograms. Superresolution by holographic superposition and synthetic aperture has a long history, which is briefly reviewed. The method is demonstrated experimentally by combining three off-axis holograms of fluorescent beads showing a transverse resolution gain of nearly a factor of 2.

© 2007 Optical Society of America

OCIS Codes
(090.0090) Holography : Holography
(110.0180) Imaging systems : Microscopy
(110.6880) Imaging systems : Three-dimensional image acquisition
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Microscopy

History
Original Manuscript: August 30, 2006
Revised Manuscript: October 13, 2006
Manuscript Accepted: October 16, 2006
Published: February 2, 2007

Virtual Issues
Vol. 2, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Guy Indebetouw, Yoshitaka Tada, Joseph Rosen, and Gary Brooker, "Scanning holographic microscopy with resolution exceeding the Rayleigh limit of the objective by superposition of off-axis holograms," Appl. Opt. 46, 993-1000 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-46-6-993

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