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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 4 — Apr. 10, 2007

Measurement of exhaled nitric oxide in beef cattle using tunable diode laser absorption spectroscopy

C. B. Roller, B. P. Holland, G. McMillen, D. L. Step, C. R. Krehbiel, K. Namjou, and P. J. McCann  »View Author Affiliations


Applied Optics, Vol. 46, Issue 8, pp. 1333-1342 (2007)
http://dx.doi.org/10.1364/AO.46.001333


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Abstract

Measurement of nitric oxide (NO) in the expired breath of crossbred calves received at a research facility was performed using tunable diode laser absorption spectroscopy. Exhaled NO (eNO) concentrations were measured using NO absorption lines at 1912.07cm1 and employing background subtraction. The lower detection limit and measurement precision were determined to be 330 parts in 1012 per unit volume. A custom breath collection system was designed to collect lower airway breath of spontaneously breathing calves while in a restraint chute. Breath was collected and analyzed from calves upon arrival and periodically during a 42 day receiving period. There was a statistically significant relationship between eNO, severity of bovine respiratory disease (BRD) in terms of number of times treated, and average daily weight gain over the first 15 days postarrival. In addition, breathing patterns and exhaled CO2 showed a statistically significant relationship with BRD morbidity.

© 2007 Optical Society of America

OCIS Codes
(140.3070) Lasers and laser optics : Infrared and far-infrared lasers
(300.1030) Spectroscopy : Absorption

History
Original Manuscript: May 5, 2006
Revised Manuscript: September 19, 2006
Manuscript Accepted: October 6, 2006
Published: February 20, 2007

Virtual Issues
Vol. 2, Iss. 4 Virtual Journal for Biomedical Optics

Citation
C. B. Roller, B. P. Holland, G. McMillen, D. L. Step, C. R. Krehbiel, K. Namjou, and P. J. McCann, "Measurement of exhaled nitric oxide in beef cattle using tunable diode laser absorption spectroscopy," Appl. Opt. 46, 1333-1342 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-46-8-1333

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