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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 6 — May. 26, 2009

Validation of an integrated Raman- and angular-scattering microscopy system on heterogeneous bead mixtures and single human immune cells

Zachary J. Smith and Andrew J. Berger  »View Author Affiliations

Applied Optics, Vol. 48, Issue 10, pp. D109-D120 (2009)

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A microscopy system has been constructed that is capable of simultaneously acquiring both Raman spectra and angle-resolved elastic-scattering patterns in either epi- or transillumination modes with a 7 μm spot size. The benefits and drawbacks of the epi- and transillumination modalities are discussed. Validation studies have been performed on single beads of a few micrometers in size, as well as on ensembles of submicrometer particles. In addition, transilluminated Raman and elastic-scattering spectra were obtained from single granulocytes and peripheral blood monocytes. Both the Raman- and the elastic-scattering channels show clear differences between the two types of immune cells.

© 2009 Optical Society of America

OCIS Codes
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(290.4020) Scattering : Mie theory

Original Manuscript: September 12, 2008
Revised Manuscript: January 5, 2009
Manuscript Accepted: January 11, 2009
Published: February 6, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

Zachary J. Smith and Andrew J. Berger, "Validation of an integrated Raman- and angular-scattering microscopy system on heterogeneous bead mixtures and single human immune cells," Appl. Opt. 48, D109-D120 (2009)

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