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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 6 — May. 26, 2009

Imaging of laser-excited tissue autofluorescence bleaching rates

Janis Spigulis, Alexey Lihachev, and Renars Erts  »View Author Affiliations

Applied Optics, Vol. 48, Issue 10, pp. D163-D168 (2009)

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Experimental methodology for imaging of laser-excited tissue autofluorescence bleaching rates has been developed and clinically tested. The fluorescence images were periodically captured from the same tissue area over a certain time, with subsequent detection of the fluorescence intensity decrease rate at each image pixel and further imaging the planar distribution of those values. Spectral features at each image pixel were analyzed with a hyperspectral imaging camera. Details of the equipment and image pro cessing are described as well as some measurement results that confirm the feasibility of the proposed technology.

© 2009 Optical Society of America

OCIS Codes
(170.1870) Medical optics and biotechnology : Dermatology
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6935) Medical optics and biotechnology : Tissue characterization

Original Manuscript: September 3, 2008
Revised Manuscript: December 11, 2008
Manuscript Accepted: December 12, 2008
Published: February 27, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

Janis Spigulis, Alexey Lihachev, and Renars Erts, "Imaging of laser-excited tissue autofluorescence bleaching rates," Appl. Opt. 48, D163-D168 (2009)

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