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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 6 — May. 26, 2009

Experimental verification of T-matrix-based inverse light scattering analysis for assessing structure of spheroids as models of cell nuclei

Cyrus Amoozegar, Michael G. Giacomelli, Justin D. Keener, Kevin J. Chalut, and Adam Wax  »View Author Affiliations

Applied Optics, Vol. 48, Issue 10, pp. D20-D25 (2009)

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Inverse light scattering analysis (ILSA) seeks to associate measured scattering properties with the most probable theoretical scattering distribution, making it a useful tool for assessing structure in biological materials. The accuracy of ILSA depends on the compatibility of the light scattering geometry with the light scattering model. In this study, we compare the accuracy obtained when analyzing light scattering data from spheroids using a numerical implementation of Mie theory, and the T matrix, a numerical method of solving light scattering from spheroids. Our experimental data are acquired using novel optical phantoms containing spheroidal scatterers and angle-resolved low-coherence interferometry, a depth- and angle-resolved light scattering measurement modality. The results show that Mie theory can accurately assess spheroidal structure despite the geometric incompatibility provided measurements are taken in multiple orientations of the sample relative to the incident polarization and the measured scattering angle. In comparison, analysis using the T-matrix method is highly accurate and more reliable yet requires measurements from only a single orientation.

© 2009 Optical Society of America

OCIS Codes
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(290.1350) Scattering : Backscattering
(290.3200) Scattering : Inverse scattering
(290.4020) Scattering : Mie theory

Original Manuscript: June 25, 2008
Revised Manuscript: November 15, 2008
Manuscript Accepted: November 24, 2008
Published: January 5, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

Cyrus Amoozegar, Michael G. Giacomelli, Justin D. Keener, Kevin J. Chalut, and Adam Wax, "Experimental verification of T-matrix-based inverse light scattering analysis for assessing structure of spheroids as models of cell nuclei," Appl. Opt. 48, D20-D25 (2009)

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