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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 6 — May. 26, 2009

Multichannel spectroreflectometry: a noninvasive method for assessment of on-line hemoglobin derivatives

Vasile Diaconu  »View Author Affiliations

Applied Optics, Vol. 48, Issue 10, pp. D52-D61 (2009)

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The goal of the current study was to introduce a mathematical method to derive hemoglobin, oxyhemoglobin and carboxyl-hemoglobin absorption factors from full spectrum reflectometry measurements of retinal microcapillaries. The mathematical equation that describes the spectral reflectometry function was expressed as a linear combination of several terms of S i ( λ ) representing the spectral signature functions of hemoglobin, oxyhemoglobin, carboxyl-hemoglobin, ocular media, melanin, and a scattering factor. Contrary to the classical model, where the reflectometry function was expressed as an absorbance Ab ( λ ) = log ( incident light ( λ ) / reflected light ( λ ) ) , in this model and system, it is proposed to express the reflectometry function from the eye structures as an absorption factor A ( λ ) % = incident light ( λ ) / reflected light ( λ ) . To increase confidence in the estimation of hemoglobin derivatives, the mathematical model was applied to only a part of the spectral function of reflectometry, while the results of the model were used to explain the other part of the reflectometry function. The results demonstrate that for the visible spectral field, the model that explains the absorption of the light by the blood contained in the microcapillaries of biological structures is not compatible with the Beer–Lambert law.

© 2009 Optical Society of America

OCIS Codes
(170.1460) Medical optics and biotechnology : Blood gas monitoring
(170.3890) Medical optics and biotechnology : Medical optics instrumentation

Original Manuscript: June 19, 2008
Revised Manuscript: November 14, 2008
Manuscript Accepted: December 5, 2008
Published: January 12, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

Vasile Diaconu, "Multichannel spectroreflectometry: a noninvasive method for assessment of on-line hemoglobin derivatives," Appl. Opt. 48, D52-D61 (2009)

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