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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 6 — May. 26, 2009

In vivo simultaneous monitoring of two fluorophores with lifetime contrast using a full-field time domain system

David J. Hall, Ulas Sunar, Salman Farshchi-Heydari, and Sung-Ho Han  »View Author Affiliations

Applied Optics, Vol. 48, Issue 10, pp. D74-D78 (2009)

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Optical molecular imaging of small animals in vivo has witnessed dramatic growth during the past decade. Most commercial systems are based on continuous wave technology and measure solely bioluminescence or fluorescence intensity. Time domain (TD) technology enables the measurement of both intensity and fluorescence lifetime as an additional imaging metric. We have developed a novel, in-house, full-field TD system with dramatically faster acquisition times than available from a commercial TD system. Recent in vivo data from a mouse imaged with the full-field TD system has demonstrated the potential to monitor and discriminate two fluorophores injected simultaneously based on their fluorescence lifetime contrast.

© 2009 Optical Society of America

OCIS Codes
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(170.7050) Medical optics and biotechnology : Turbid media

Original Manuscript: September 3, 2008
Revised Manuscript: December 1, 2008
Manuscript Accepted: December 16, 2008
Published: January 21, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

David J. Hall, Ulas Sunar, Salman Farshchi-Heydari, and Sung-Ho Han, "In vivo simultaneous monitoring of two fluorophores with lifetime contrast using a full-field time domain system," Appl. Opt. 48, D74-D78 (2009)

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