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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 6 — May. 26, 2009

Following dimethyl sulfoxide skin optical clearing dynamics with quantitative nonlinear multimodal microscopy

Maxwell Zimmerley, R. Anthony McClure, Bernard Choi, and Eric Olaf Potma  »View Author Affiliations


Applied Optics, Vol. 48, Issue 10, pp. D79-D87 (2009)
http://dx.doi.org/10.1364/AO.48.000D79


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Abstract

Second-harmonic generation (SHG) imaging is combined with coherent anti-Stokes Raman scattering (CARS) microscopy to follow the process of optical clearing in human skin ex vivo using dimethyl sulfoxide (DMSO) as the optical clearing agent. SHG imaging revealed that DMSO introduces morphological changes to the collagen I matrix. By carefully measuring the dynamic tissue attenuation of the coherent nonlinear signal, using CARS reference signals during the clearing process, it is found that DMSO reduces the overall SHG response from dermal collagen. Evidence is provided for a role of DMSO in compromising the structure of collagen fibers, associated with a reduction of the tissue’s scattering properties.

© 2009 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.4315) Microscopy : Nonlinear microscopy

History
Original Manuscript: September 5, 2008
Revised Manuscript: December 11, 2008
Manuscript Accepted: December 19, 2008
Published: January 21, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Maxwell Zimmerley, R. Anthony McClure, Bernard Choi, and Eric Olaf Potma, "Following dimethyl sulfoxide skin optical clearing dynamics with quantitative nonlinear multimodal microscopy," Appl. Opt. 48, D79-D87 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-48-10-D79

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