Ex vivo and in vivo second-harmonic-generation imaging of dermal collagen fiber in skin: comparison of imaging characteristics between mode-locked Cr:forsterite and Ti:sapphire lasers
Applied Optics, Vol. 48, Issue 10, pp. D88-D95 (2009)
http://dx.doi.org/10.1364/AO.48.000D88
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Abstract
Second-harmonic-generation (SHG) microscopy is an interesting new tool for observing dermal collagen fiber in skin. However, conventional SHG microscopy using a mode-locked Ti:sapphire laser suffers from low penetration depth and a slow image acquisition rate caused by scattering and absorption in tissue, making it difficult to use for in vivo applications on human skin. We develop an SHG microscope equipped with a mode-locked Cr:forsterite laser with a long wavelength and compare its imaging characteristics with that of a Ti:sapphire-laser-based SHG microscope for the measurement of dermal collagen fiber in animal and human skins. The results indicate the suitability of the Cr:forsterite laser-based SHG microscope for in vivo imaging of human skin.
© 2009 Optical Society of America
OCIS Codes
(170.1870) Medical optics and biotechnology : Dermatology
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6960) Medical optics and biotechnology : Tomography
(190.4160) Nonlinear optics : Multiharmonic generation
(180.4315) Microscopy : Nonlinear microscopy
History
Original Manuscript: September 2, 2008
Revised Manuscript: December 22, 2008
Manuscript Accepted: December 26, 2008
Published: January 26, 2009
Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics
Citation
Takeshi Yasui, Yu Takahashi, Masahiro Ito, Shuichiro Fukushima, and Tsutomu Araki, "Ex vivo and in vivo second-harmonic-generation imaging of dermal collagen fiber in skin: comparison of imaging characteristics between mode-locked Cr:forsterite and Ti:sapphire lasers," Appl. Opt. 48, D88-D95 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-48-10-D88
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