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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 8 — Jul. 30, 2009

Simple spatial phase modulator for focal modulation microscopy

Chee Howe Wong, Shau Poh Chong, Colin J. R. Sheppard, and Nanguang Chen  »View Author Affiliations


Applied Optics, Vol. 48, Issue 17, pp. 3237-3242 (2009)
http://dx.doi.org/10.1364/AO.48.003237


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Abstract

Focal modulation microscopy (FMM) is an emerging microscopy technique for fluorescence imaging of thick biological tissue in vivo. A spatial phase modulator is a critical component whose characteristics have a significant impact on the performance of a FMM system. We have designed a simple spatial phase modulator based on a tilting glass plate that provides superb modulation stability. Image quality has been improved remarkably after integrating such a modulator into a FMM system.

© 2009 Optical Society of America

OCIS Codes
(120.5060) Instrumentation, measurement, and metrology : Phase modulation
(180.2520) Microscopy : Fluorescence microscopy
(230.4110) Optical devices : Modulators
(110.0113) Imaging systems : Imaging through turbid media

ToC Category:
Microscopy

History
Original Manuscript: February 18, 2009
Revised Manuscript: May 19, 2009
Manuscript Accepted: May 20, 2009
Published: June 8, 2009

Virtual Issues
Vol. 4, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Chee Howe Wong, Shau Poh Chong, Colin J. R. Sheppard, and Nanguang Chen, "Simple spatial phase modulator for focal modulation microscopy," Appl. Opt. 48, 3237-3242 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-48-17-3237

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