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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 10 — Oct. 2, 2009

Surface-enhanced Raman scattering biosensor for DNA detection on nanoparticle island substrates

Wu Yuan, Ho Pui Ho, Rebecca K. Y. Lee, and Siu Kai Kong  »View Author Affiliations

Applied Optics, Vol. 48, Issue 22, pp. 4329-4337 (2009)

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We present a study on the surface-enhanced Raman scattering (SERS) properties of Ag nanoparticle island substrates (NIS) and their applications for target oligonucleotide (OND) detection. It has been found that the surface nanostructure of NIS samples can be controlled with a good degree of reproducibility, and a high SERS enhancement can be achieved when the peak extinction wavelength of NIS is tuned to a spectral window ( 60 nm ) between the excitation wavelength and the scattered Raman wavelength. The highest SERS enhancement was obtained from the NIS substrates with a nominal thickness of 50 Å . Detection of target OND was performed with a sandwich format in which the target OND was hybridized both to a capture OND immobilized on the NIS substrate, and a detection OND conjugated with a Raman-active dye for SERS signal generation. We compare the detection performance of two strategies based on the use of the detection OND with or without the gold nanoparticle (Au-NP). Our results confirm that, when the detection OND is coupled to the Au-NP, a better sensitivity for the target OND detection, in terms of a wider dynamic range and a lower detection limit ( 0.4 fM versus 1 nM without Au-NP), would be achieved.

© 2009 Optical Society of America

OCIS Codes
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(240.6680) Optics at surfaces : Surface plasmons
(300.6450) Spectroscopy : Spectroscopy, Raman
(240.6695) Optics at surfaces : Surface-enhanced Raman scattering

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: March 9, 2009
Revised Manuscript: June 12, 2009
Manuscript Accepted: June 16, 2009
Published: July 22, 2009

Virtual Issues
Vol. 4, Iss. 10 Virtual Journal for Biomedical Optics
July 23, 2009 Spotlight on Optics

Wu Yuan, Ho Pui Ho, Rebecca K. Y. Lee, and Siu Kai Kong, "Surface-enhanced Raman scattering biosensor for DNA detection on nanoparticle island substrates," Appl. Opt. 48, 4329-4337 (2009)

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