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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 12 — Nov. 10, 2009

Quantification of the optical properties of two-layer turbid materials using a hyperspectral imaging-based spatially-resolved technique

Haiyan Cen and Renfu Lu  »View Author Affiliations


Applied Optics, Vol. 48, Issue 29, pp. 5612-5623 (2009)
http://dx.doi.org/10.1364/AO.48.005612


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Abstract

Recent research has shown that a hyperspectral imaging-based spatially-resolved technique is useful for determining the optical properties of homogenous fruits and food products. To better characterize fruit properties and quality attributes, it is desirable to consider fruit to be composed of two homogeneous layers of skin and flesh. This research was aimed at developing a nondestructive method to determine the absorption and scattering properties of two-layer turbid materials with the characteristics of fruit. An inverse algorithm along with the sensitivity coefficient analysis for a two-layer diffusion model was developed for the extraction of optical properties from the spatially-resolved diffuse reflectance data acquired using a hyperspectral imaging system. The diffusion model and the inverse algorithm were validated with Monte Carlo simulations and experimental measurements from solid model samples of known optical properties. The average errors of determining two and four optical parameters were 6.8% and 15.3%, respectively, for Monte Carlo reflectance data. The optical properties of the first or top layer of the model samples were determined with errors of less than 23.0% for the absorption coefficient and 18.4% for the reduced scattering coefficient. The inverse algorithm did not give acceptable estimations for the second or lower layer of the model samples. While the hyperspectral imaging-based spatially-resolved technique has the potential to measure the optical properties of two-layer turbid materials like fruits and food products, further improvements are needed in determining the optical properties of the second layer.

© 2009 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.7050) Medical optics and biotechnology : Turbid media
(290.0290) Scattering : Scattering
(300.1030) Spectroscopy : Absorption

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: July 2, 2009
Revised Manuscript: September 8, 2009
Manuscript Accepted: September 10, 2009
Published: October 7, 2009

Virtual Issues
Vol. 4, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Haiyan Cen and Renfu Lu, "Quantification of the optical properties of two-layer turbid materials using a hyperspectral imaging-based spatially-resolved technique," Appl. Opt. 48, 5612-5623 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-48-29-5612

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