Lorentzian spatial intensity distribution in one-photon fluorescence correlation spectroscopy
Applied Optics, Vol. 48, Issue 31, pp. 6050-6058 (2009)
http://dx.doi.org/10.1364/AO.48.006050
Enhanced HTML 
Acrobat PDF (688 KB)
Abstract
The theory of autocorrelation-function evaluation in fluorescence correlation spectroscopy is applied to a Lorentzian intensity distribution. An analytical solution to the autocorrelation function for diffusion is deduced for this spatial distribution. Experimental investigation of the distribution is performed using an enlarged detector aperture in a standard confocal setup. The data from the experiment are fitted to the derived autocorrelation function, and a reasonable estimate of the spatial distribution is provided. Estimates are also compared to values computed by molecular detection efficiency simulation. The use of Lorentzian intensity distributions complements conditions where a Gaussian intensity distribution applies, expanding the applicability range of analytical correlation functions.
© 2009 Optical Society of America
OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(300.2530) Spectroscopy : Fluorescence, laser-induced
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: July 22, 2009
Revised Manuscript: September 25, 2009
Manuscript Accepted: October 8, 2009
Published: October 28, 2009
Virtual Issues
Vol. 4, Iss. 13 Virtual Journal for Biomedical Optics
Citation
Hans Blom and Gunnar Björk, "Lorentzian spatial intensity distribution in one-photon fluorescence correlation spectroscopy," Appl. Opt. 48, 6050-6058 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-48-31-6050
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Monte Carlo Simulation of a Single-Molecule Detection Experiment (AO)
- Fiber-Optic Bundle Design for Quantitative Fluorescence Measurement From Tissue (AO)
- Thin-Film Luminescence under Ultraviolet Irradiation (AO)
- Spatial fluorescence cross-correlation spectroscopy (AO)
- Parallel confocal detection of single molecules in real time (OL)
Related Conference Papers
- Multiple UV wavelength excitation and fluorescence of bioaerosols
- Multi-Channel Imaging Capabilities at Low Signal Levels
- Single Molecule Pulsed Interleaved Excitation Fluorescence Resonance Energy Transfer (PIE-FRET) inside Nanometer-Scale Apertures at Biologically Relevant Concentration
- Development of a New Sensitive and Rapid Detection Method of Specific DNA Sequences
- Microfluidic Device for the 3-D Electrokinetic Manipulation of Single Molecules
« Previous Article | Next Article »
OSA is a member of 