Determination of cell elasticity through hybrid ray optics and continuum mechanics modeling of cell deformation in the optical stretcher
Applied Optics, Vol. 48, Issue 32, pp. 6344-6354 (2009)
http://dx.doi.org/10.1364/AO.48.006344
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Abstract
The optical stretcher is a dual-beam trap capable of stretching individual cells. Previous studies have used either ray- or wave-optical models to compute the optical pressure on the surface of a spherical cell. We have extended the ray-optics model to account for focusing by the spherical interface and the effects of multiple internal reflections. Simulation results for red-blood cells (RBCs) show that internal reflections can lead to significant perturbation of the deformation, leading to a systematic error in the determination of cellular elasticity. Calibration studies show excellent agreement between the predicted and measured escape force, and RBC stiffness measurements are consistent with literature values. Measurements of the elasticity of murine osteogenic cells reveal that these cells are approximately 5.4 times stiffer than RBCs.
© 2009 Optical Society of America
OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: June 9, 2009
Revised Manuscript: September 14, 2009
Manuscript Accepted: October 11, 2009
Published: November 6, 2009
Virtual Issues
Vol. 4, Iss. 13 Virtual Journal for Biomedical Optics
Citation
Andrew E. Ekpenyong, Carolyn L. Posey, Joy L. Chaput, Anya K. Burkart, Meg M. Marquardt, Timothy J. Smith, and Michael G. Nichols, "Determination of cell elasticity through hybrid ray optics and continuum mechanics modeling of cell deformation in the optical stretcher," Appl. Opt. 48, 6344-6354 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-48-32-6344
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