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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 4 — Apr. 1, 2009

Optical waveguide light-mode spectroscopy immunosensors for environmental monitoring

András Székács, Nóra Adányi, Inna Székács, Krisztina Majer-Baranyi, and István Szendrő  »View Author Affiliations

Applied Optics, Vol. 48, Issue 4, pp. B151-B158 (2009)

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Coupling the high specificity of the immunoanalytical reaction with the high sensitivity of optical wave guide light-mode spectroscopy (OWLS) detection gives the possibility to develop immunosensors with in most cases a definitely lower detection limit than traditionally used immunoassays. Measurements were performed on the sensitized surface of optical waveguide grating coupler sensors ( 2400   lines /mm grating). The OWLS technique is based on the precise measurement of the resonance angle of a polarized laser light ( 632.8 nm ), diffracted by a grating and incoupled into a thin waveguide. The effective refractive index, determined from the resonance incoupling angle detected at high accuracy, allows determination of layer thickness and coverage (or mass) of the adsorbed or bound material with ultrahigh sensitivity. OWLS immunosensors were developed as label-free immunosensors with an amino group modified SiO 2 - TiO 2 sensor surface on which the immunoreactants could be anchored. One of the components of the antibody–antigen complex was chemically bound on the sensor surface, allowing noncompetitive or competitive detection of the analytes. To illustrate that the resulting immunosensors are suitable for the determination of small and large molecular weight analytes, OWLS sensor formats were applied for quantitative detection of a herbicide active ingredient trifluralin, a Fusarium mycotoxin zearalenone, and an egg yolk protein of key importance in endocrine regulation, vitellogenin.

© 2009 Optical Society of America

OCIS Codes
(120.4820) Instrumentation, measurement, and metrology : Optical systems
(130.6010) Integrated optics : Sensors
(230.7370) Optical devices : Waveguides
(350.2770) Other areas of optics : Gratings
(130.2755) Integrated optics : Glass waveguides
(280.4788) Remote sensing and sensors : Optical sensing and sensors

Original Manuscript: July 31, 2008
Revised Manuscript: November 17, 2008
Manuscript Accepted: November 25, 2008
Published: January 8, 2009

Virtual Issues
Vol. 4, Iss. 4 Virtual Journal for Biomedical Optics

András Székács, Nóra Adányi, Inna Székács, Krisztina Majer-Baranyi, and István Szendrő, "Optical waveguide light-mode spectroscopy immunosensors for environmental monitoring," Appl. Opt. 48, B151-B158 (2009)

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