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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 4 — Apr. 1, 2009

Distributed feedback diode laser spectrometer at 2.7 μm for sensitive, spatially resolved H 2 O vapor detection

Karl Wunderle, Steven Wagner, Igor Pasti, Roland Pieruschka, Uwe Rascher, Ulrich Schurr, and Volkert Ebert  »View Author Affiliations


Applied Optics, Vol. 48, Issue 4, pp. B172-B182 (2009)
http://dx.doi.org/10.1364/AO.48.00B172


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Abstract

A new, compact, spatially scanning, open-path 2.7 μm tunable diode laser absorption spectrometer with short absorption path lengths below 10 cm was developed to analyze the spatiotemporal dynamics of one-dimensional (1D) spatial water vapor gradients. This spectrometer, which is based on a room- temperature distributed feedback diode laser, is capable of measuring absolute, calibration-free, line-of-sight averaged, but laterally resolved 1D H 2 O concentration profiles with a minimum fractional optical resolution of 2.1 × 10 3 optical density (OD) ( 2.5 × 10 4 OD after a background subtraction procedure), which permits a signal-to-noise-ratio of 407 (3400) at 10 , 000 parts in 10 6 ( ppm ) H 2 O , or normalized sensitivities of 2.6 ppm m ( 0.32 ppm m ) at 0.5 Hz duty cycle. The spectrometer’s lateral spatial resolution (governed by the 500 μm sampling beam diameter) was validated by analyzing a well-defined laminar jet of nitrogen gas in humidified air. This scanning setup was then used to (a) quantitatively investigate for what we believe to be the first time the H 2 O boundary layer from 0.7 to 11 mm beneath the stomatous side of a single, undetached plant leaf, and (b) to study the temporal boundary layer dynamics and its dependence on stepwise light stimulation of the photosynthetic system. In addition the 2.7 μm diode laser was carefully characterized in terms of spectral purity, beam profile, as well as quasi-static and dynamic wavelength tuning coefficients.

© 2009 Optical Society of America

OCIS Codes
(120.6200) Instrumentation, measurement, and metrology : Spectrometers and spectroscopic instrumentation
(300.6260) Spectroscopy : Spectroscopy, diode lasers
(300.6340) Spectroscopy : Spectroscopy, infrared

History
Original Manuscript: August 19, 2008
Revised Manuscript: December 12, 2008
Manuscript Accepted: December 17, 2008
Published: January 16, 2009

Virtual Issues
Vol. 4, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Karl Wunderle, Steven Wagner, Igor Pasti, Roland Pieruschka, Uwe Rascher, Ulrich Schurr, and Volkert Ebert, "Distributed feedback diode laser spectrometer at 2.7 μm for sensitive, spatially resolved H2O vapor detection," Appl. Opt. 48, B172-B182 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-48-4-B172

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