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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 9 — Jul. 6, 2010

Time-resolved fluorescence spectroscopy of white-spot caries in human enamel

Fernanda Ferretti de Oliveira, Amando Siuiti Ito, and Luciano Bachmann  »View Author Affiliations


Applied Optics, Vol. 49, Issue 12, pp. 2244-2249 (2010)
http://dx.doi.org/10.1364/AO.49.002244


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Abstract

The objective is to differentiate noncavitated caries enamel through time-resolved fluorescence and to find excitation and emission parameters that can be applied in future clinical practice for detection of caries lesions that are not clearly visible to the professional. Sixteen human teeth with noncavitiated white-spot caries were selected for this work. Fluorescence intensity decay was measured by using an apparatus based on the time-correlated single-photon counting method. An optical fiber bundle was employed for sample excitation ( 440 nm ), and the fluorescence collected by the same bundle ( 500 nm ) was registered. The average lifetime for sound enamel was 7.93 ± 0.09 , 2.46 ± 0.04 , and 0.51 ± 0.02 ns , whereas for the carious enamel the lifetimes were 4.84 ± 0.06 , 1.35 ± 0.02 , and 0.16 ± 0.01 ns . It was concluded that it is possible to differentiate between carious and sound regions by time-resolved fluorescence and that, although the origin of enamel fluorescence is still uncertain, the lifetime values seem to be typical of fluorophores like collagen I.

© 2010 Optical Society of America

OCIS Codes
(140.0140) Lasers and laser optics : Lasers and laser optics
(300.2530) Spectroscopy : Fluorescence, laser-induced

ToC Category:
Spectroscopy

History
Original Manuscript: November 20, 2009
Manuscript Accepted: March 4, 2010
Published: April 13, 2010

Virtual Issues
Vol. 5, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Fernanda Ferretti de Oliveira, Amando Siuiti Ito, and Luciano Bachmann, "Time-resolved fluorescence spectroscopy of white-spot caries in human enamel," Appl. Opt. 49, 2244-2249 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-49-12-2244


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