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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 9 — Jul. 6, 2010

Time-resolved fluorescence spectroscopy of white-spot caries in human enamel

Fernanda Ferretti de Oliveira, Amando Siuiti Ito, and Luciano Bachmann  »View Author Affiliations

Applied Optics, Vol. 49, Issue 12, pp. 2244-2249 (2010)

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The objective is to differentiate noncavitated caries enamel through time-resolved fluorescence and to find excitation and emission parameters that can be applied in future clinical practice for detection of caries lesions that are not clearly visible to the professional. Sixteen human teeth with noncavitiated white-spot caries were selected for this work. Fluorescence intensity decay was measured by using an apparatus based on the time-correlated single-photon counting method. An optical fiber bundle was employed for sample excitation ( 440 nm ), and the fluorescence collected by the same bundle ( 500 nm ) was registered. The average lifetime for sound enamel was 7.93 ± 0.09 , 2.46 ± 0.04 , and 0.51 ± 0.02 ns , whereas for the carious enamel the lifetimes were 4.84 ± 0.06 , 1.35 ± 0.02 , and 0.16 ± 0.01 ns . It was concluded that it is possible to differentiate between carious and sound regions by time-resolved fluorescence and that, although the origin of enamel fluorescence is still uncertain, the lifetime values seem to be typical of fluorophores like collagen I.

© 2010 Optical Society of America

OCIS Codes
(140.0140) Lasers and laser optics : Lasers and laser optics
(300.2530) Spectroscopy : Fluorescence, laser-induced

ToC Category:

Original Manuscript: November 20, 2009
Manuscript Accepted: March 4, 2010
Published: April 13, 2010

Virtual Issues
Vol. 5, Iss. 9 Virtual Journal for Biomedical Optics

Fernanda Ferretti de Oliveira, Amando Siuiti Ito, and Luciano Bachmann, "Time-resolved fluorescence spectroscopy of white-spot caries in human enamel," Appl. Opt. 49, 2244-2249 (2010)

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  1. I. A. Pretty, “Caries detection and diagnosis: novel technologies,” J. Dent. Res. 34, 727-739 (2006). [CrossRef]
  2. S. M Higham, N. Pender, E. J Jong, and P. W. Smith, “Application of biophysical technologies in dental research,” J. Appl. Phys. 105, 102048 (2009). [CrossRef]
  3. V. Baelum, J. Heidmann, and B. Nyvad, “Dental caries paradigms in diagnosis and diagnostic research,” Eur. J. Oral Sci. 114, 263-277 (2006). [CrossRef] [PubMed]
  4. B. T. Amaechi, “Emerging technologies for diagnosis of dental caries: the road so far,” J. Appl. Phys. 105, 102047(2009). [CrossRef]
  5. S. Tranaeus, X-Q. Shi, and B. Angmar-Manssson, “Caries risk assessment: methods available to clinicians for caries detection,” Community Dent. Oral Epidemiol. 33, 265-273(2005). [CrossRef] [PubMed]
  6. A. Lussi, R. Hibst, and R. Paulus, “DIAGNOdent: an optical method for caries detection,” J. Dent. Res. 83, C80-C83(2004). [CrossRef] [PubMed]
  7. W. Buchalla, “Comparative fluorescence spectroscopy shows differences in noncavitated enamel lesions,” Caries Res. 39, 150-156 (2005). [CrossRef] [PubMed]
  8. L. Bachmann, D. M. Zezell, A. C. Ribeiro, L. Gomes, and A. S. Ito, “Fluorescence of biological tissue: a review,” Appl. Spectrosc. Rev. 41, 575-590 (2006). [CrossRef]
  9. R. R. Alfano and S. S. Yao, “Human teeth with and without dental caries studied by visible luminescence spectroscopy,” J. Dent. Res. 60, 120-122 (1981). [CrossRef] [PubMed]
  10. K. König, H. Schneckenburguer, and R. Hibst, “Time-gated in vivo autofluorescence imaging of dental caries,” Cell. Mol. Biol. (Paris) 45, 233-239 (1999).
  11. H. Schneckenburguer and K. Konig, “Fluorescence decay kinetics and imaging of NAD(P)H and flavins as metabolic indicators,” Opt. Eng. 31, 1447-1451 (1992). [CrossRef]
  12. Q Fang, T. Papaioannou, J. A. Jo, R. Vaitha, K. Shastry, and L. Marcu, “Time-domain laser-induced fluorescence spectroscopy apparatus for clinical diagnostics,” Rev. Sci. Instrum. 75, 151-162 (2004) [CrossRef]
  13. L. Marcu, D. Cohen, J. I. Maarek, and W. S. Grundfest, “Characterization of type I, II, III, IV, and V collagens by time-resolved laser-induced fluorescence spectroscopy,” Proc. SPIE 3917, 93-101 (2000). [CrossRef]
  14. C. Robinson, R. C. Shore, S. J. Brookes, S. Strafford, S. R. Wood, and J. Kirkham, “The chemistry of enamel caries,” Crit. Rev. Oral Biol. Med. 11, 481-495 (2000). [CrossRef]
  15. K. Konig, G. Flemming, and R. Hibst, “Laser-induced autofluorescence spectroscopy of dental caries,” Cell. Mol. Biol. (Paris) 44, 1293-1300 (1998).
  16. D. M. Zezell, A. C. Ribeiro, L. Bachmann, A. S. L. Gomes, C. Rousseau, and J. Girkin, “Characterization of natural carious lesions by fluorescence spectroscopy at 405 nm excitation wavelength,” J. Biomed. Opt. 12, 064013 (2007). [CrossRef]
  17. A. C. R. Figueiredo, C. Kurachi, and V. S. Bagnato, “Comparison of fluorescence detection of carious dentin for different excitation wavelenghts,” Caries Res. 39, 393-396(2005). [CrossRef]

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